Algicidal effects of Zostera marina L. and Zostera noltii Hornem. extracts on the neuro-toxic bloom-forming dinoflagellate Alexandrium catenella

Type Article
Date 2013-11
Language English
Author(s) Laabir Mohamed1, Grignon-Dubois Micheline2, Masseret Estelle1, Rezzonico Bernadette2, Soteras Giselle3, Rouquette Manuel1, Rieuvilleneuve Fabien1, Cecchi Philippe
Affiliation(s) 1 : Univ Montpellier 2, Ecol Syst Marine Cotiers UMR5119, IFREMER, CNRS,UM2,IRD,UM1, F-34095 Montpellier 5, France.
2 : Univ Bordeaux, UMR EPOC 5805, F-33405 Talence, France.
3 : Conseil Gen Dept Herault, F-34087 Montpellier, France.
Source Aquatic Botany (0304-3770) (Elsevier Science Bv), 2013-11 , Vol. 111 , P. 16-25
DOI 10.1016/j.aquabot.2013.07.010
WOS© Times Cited 22
Keyword(s) Growth inhibition, Allelopathy, Alexandrium catenella, Zostera marina, Zostera noltii, Phenolics
Abstract The inhibitory effects of crude extracts of Zostera marina L and Zostera noltii Hornemann on the growth of the toxic red tide dinoflagellate Alexandrium catenella were investigated through bio-assays. Methanolic and aqueous extracts were prepared from fresh and detrital leaves of Z. marina and Z. noltii collected in the Thau lagoon and Arcachon bay (France). All the extracts exhibited significant inhibition of A. catenella growth, whatever the species and without the need of continuous addition of extracts. The effective concentrations (EC50) varied in the range 0.036-0.199 g L-1 for Z. noltii and 0.036-0.239 g L-1 for Z. marina. Methanolic extracts prepared from fresh plant tissues were the most potent, with EC50 of 0.036-0.039 and 0.036-0.045 g L-1, for Z. noltii and Z marina, respectively. After exposure to the extracts, Alexandrium cells exhibited severe morphological anomalies. Hence many cells exhibited a lytic membrane and became necrotic. Cellular degradation appeared dose- and time-dependent. The observed algicidal activities demonstrated the existence of bioactive molecules in the tissues of Zostera leaves, which were stable in the culture medium. The chemical contents of the crude extracts were determined by NMR, LC/MS, and quantitative HPLC. Results showed the predominance of flavonoids and phenolic acids. The total phenolics concentrations correlated negatively with the EC50 values, suggesting that these secondary metabolites might be responsible for the observed algicidal effects.
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Laabir Mohamed, Grignon-Dubois Micheline, Masseret Estelle, Rezzonico Bernadette, Soteras Giselle, Rouquette Manuel, Rieuvilleneuve Fabien, Cecchi Philippe (2013). Algicidal effects of Zostera marina L. and Zostera noltii Hornem. extracts on the neuro-toxic bloom-forming dinoflagellate Alexandrium catenella. Aquatic Botany, 111, 16-25. Publisher's official version : https://doi.org/10.1016/j.aquabot.2013.07.010 , Open Access version : https://archimer.ifremer.fr/doc/00170/28095/