FN Archimer Export Format
PT J
TI Somatotropic axis genes are expressed before pituitary onset during zebrafish and sea bass development
BT 
AF BESSEAU, Laurence
   FUENTES, Michael
   SAUZET, Sandrine
   BEAUCHAUD, Marilyn
   CHATAIN, Beatrice
   COVES, Denis
   BOEUF, Gilles
   FALCON, Jack
AS 1:1,2;2:1,2;3:1,2;4:3;5:4;6:4;7:1,2,5;8:1,2;
FF 1:;2:;3:;4:;5:PDG-RBE-BOME-LALR;6:PDG-RBE-BOME-LALR;7:;8:;
C1 Univ Paris 06, Lab Arago, F-66650 Banyuls Sur Mer, France.
   CNRS UMR 7232, F-66650 Banyuls Sur Mer, France.
   Univ Lyon St Etienne, Equipe Neuroethol Sensorielle, ENES CNPS, CNRS UMR 8195, F-42023 St Etienne 2, France.
   Stn Ifremer Palavas, UMR Intrepid 110, F-34250 Palavas Les Flots, France.
   MNHN, F-75005 Paris, France.
C2 UNIV PARIS 06, FRANCE
   CNRS, FRANCE
   UNIV ST ETIENNE, FRANCE
   IFREMER, FRANCE
   MNHN, FRANCE
SI PALAVAS
SE PDG-RBE-BOME-LALR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.674
TC 15
UR https://archimer.ifremer.fr/doc/00170/28100/88182.pdf
LA English
DT Article
DE ;Growth hormone GH;Insulin-like growth factor-1 IGF-1;Pituitary;Development;Zebrafish;Sea bass
AB The somatotropic axis, or growth hormone-insulin-like growth factor-1 (GH-IGF-1) axis, of fish is involved in numerous physiological process including regulation of ionic and osmotic balance, lipid, carbohydrate and protein metabolism, growth, reproduction, immune function and behavior. It is thought that GH plays a role in fish development but conflicting results have been obtained concerning the ontogeny of the somatotropic axis. Here we investigated the developmental expression of GH, GH-receptor (GHR) and IGF-1 genes and of a GH-like protein from fertilization until early stages of larval development in two Teleosts species, Danio rerio and Dicentrarchus labrax, by PCR, in situ hybridization and Western blotting. GH, GHR and IGF-1 mRNA were present in unfertilized eggs and at all stages of embryonic development, all three displaying a similar distribution in the two species. First located in the whole embryo (until 12 hpf in zebrafish and 76 hpf in sea bass), the mRNAs appeared then distributed in the head and tail, from where they disappeared progressively to concentrate in the forming pituitary gland. Proteins immunoreactive with a specific sea bass anti-GH antibody were also detected at all stages in this species. Differences in intensity and number of bands suggest that protein processing varies from early to later stages of development. The data show that all actors of the somatotropic axis are present from fertilization in these two species, suggesting they plays a role in early development, perhaps in an autocrine/paracrine mode as all three elements displayed a similar distribution at each stage investigated.
PY 2013
PD DEC
SO General And Comparative Endocrinology
SN 0016-6480
PU Academic Press Inc Elsevier Science
VL 194
UT 000328006600015
BP 133
EP 141
DI 10.1016/j.ygcen.2013.08.018
ID 28100
ER

EF