FN Archimer Export Format
PT J
TI An Extended Network of Genomic Maintenance in the Archaeon Pyrococcus abyssi Highlights Unexpected Associations between Eucaryotic Homologs
BT 
AF PLUCHON, Pierre Francois
   FOUQUEAU, Thomas
   CREZE, Christophe
   LAURENT, Sebastien
   BRIFFOTAUX, Julien
   HOGREL, Gaelle
   PALUD, Adeline
   HENNEKE, Ghislaine
   GODFROY, Anne
   HAUSNER, Winfried
   THOMM, Michael
   NICOLAS, Jacques
   FLAMENT, Didier
AS 1:1,2,3;2:4;3:1,2,3;4:1,2,3;5:1,2,3;6:1,2,3;7:1,2,3;8:1,2,3;9:1,2,3;10:4;11:4;12:5;13:1,2,3;
FF 1:;2:;3:PDG-REM-EEP-LMEE;4:PDG-REM-EEP-LMEE;5:PDG-REM-EEP-LMEE;6:;7:PDG-REM-EEP-LMEE;8:PDG-REM-EEP-LMEE;9:PDG-REM-EEP-LMEE;10:;11:;12:;13:PDG-REM-EEP-LMEE;
C1 IFREMER, Lab Microbiol Environm Extremes, UMR6197, Plouzane, France.
   Univ Bretagne Occidentale, Lab Microbiol Environm Extremes, UMR6197, Plouzane, France.
   CNRS, Lab Microbiol Environm Extremes, UMR6197, Plouzane, France.
   Univ Regensburg, Lehrstuhl Mikrobiol, D-93053 Regensburg, Germany.
   IRISA INRIA, Rennes, France.
C2 IFREMER, FRANCE
   UBO, FRANCE
   CNRS, FRANCE
   UNIV REGENSBURG, GERMANY
   IRISA INRIA, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 3.534
TC 22
UR https://archimer.ifremer.fr/doc/00171/28198/26556.pdf
LA English
DT Article
AB In Archaea, the proteins involved in the genetic information processing pathways, including DNA replication, transcription, and translation, share strong similarities with those of eukaryotes. Characterizations of components of the eukaryotic-type replication machinery complex provided many interesting insights into DNA replication in both domains. In contrast, DNA repair processes of hyperthermophilic archaea are less well understood and very little is known about the intertwining between DNA synthesis, repair and recombination pathways. The development of genetic system in hyperthermophilic archaea is still at a modest stage hampering the use of complementary approaches of reverse genetics and biochemistry to elucidate the function of new candidate DNA repair gene. To gain insights into genomic maintenance processes in hyperthermophilic archaea, a protein-interaction network centred on informational processes of Pyrococcus abyssi was generated by affinity purification coupled with mass spectrometry. The network consists of 132 interactions linking 87 proteins. These interactions give insights into the connections of DNA replication with recombination and repair, leading to the discovery of new archaeal components and of associations between eucaryotic homologs. Although this approach did not allow us to clearly delineate new DNA pathways, it provided numerous clues towards the function of new molecular complexes with the potential to better understand genomic maintenance processes in hyperthermophilic archaea. Among others, we found new potential partners of the replication clamp and demonstrated that the single strand DNA binding protein, Replication Protein A, enhances the transcription rate, in vitro, of RNA polymerase. This interaction map provides a valuable tool to explore new aspects of genome integrity in Archaea and also potentially in Eucaryotes.
PY 2013
PD NOV
SO Plos One
SN 1932-6203
PU Public Library Science
VL 8
IS 11
UT 000327162900046
DI 10.1371/journal.pone.0079707
ID 28198
ER

EF