TY - JOUR T1 - Kosmotoga pacifica sp. nov., a thermophilic chemoorganoheterotrophic bacterium isolated from an East Pacific hydrothermal sediment A1 - L'Haridon,Stephane A1 - Jiang,Lijing A1 - Alain,Karine A1 - Chalopin,Morgane A1 - Rouxel,Ouafae A1 - Beauverger,Mikael A1 - Xu,Hongxiu A1 - Shao,Zongze A1 - Jebbar,Mohamed AD - Univ Bretagne Occidentale, IUEM, LMEE, UEB,UMR 6197, F-29280 Plouzane, France. AD - CNRS, IUEM, LMEE, UMR 6197, F-29280 Plouzane, France. AD - IFREMER, LMEE, UMR 6197, F-29280 Plouzane, France. AD - SOA, Inst Oceanog 3, Key Lab Marine Biogenet Resources, Xiamen 361005, Peoples R China. UR - https://doi.org/10.1007/s00792-013-0596-7 DO - 10.1007/s00792-013-0596-7 KW - Thermotogales KW - Kosmotoga KW - Kosmotoga pacifica KW - Hydrothermal sediment N2 - A novel strictly anaerobic thermophilic heterotrophic bacterium, strain SLHLJ1T, was isolated from a Pacific hydrothermal sediment. Cells were Gram-negative coccobacilli (approximately 1.0 × 0.6 μm) with a toga. It grew at temperatures between 33 and 78 °C (optimum 70 °C). Elemental sulphur and l-cystine stimulated its growth. It contained C16:0, C16:1 ω11c, C18:0 and C18:1 ω9c as major fatty acids (>5 %), 3 phospholipids and 2 glycolipids as polar lipids. Its DNA G+C content was 43.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLHLJ1T within the family Thermotogaceae. The novel isolate was most closely related to Kosmotoga arenicorallina (97.93 % 16S rRNA gene sequence similarity), K. olearia (92.43 %) and K. shengliensis (92.17 %). On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons with its closest relatives, we propose its assignment to a novel species of the genus Kosmotoga. The name Kosmotoga pacifica sp. nov. is proposed with strain SLHLJ1T (=DSM 26965T = JCM 19180T = UBOCC 3254T) as the type species. Y1 - 2014/01 PB - Springer Japan Kk JF - Extremophiles SN - 1431-0651 VL - 18 IS - 1 SP - 81 EP - 88 ID - 28204 ER -