FN Archimer Export Format PT J TI Kosmotoga pacifica sp. nov., a thermophilic chemoorganoheterotrophic bacterium isolated from an East Pacific hydrothermal sediment BT AF L'HARIDON, Stephane JIANG, Lijing ALAIN, Karine CHALOPIN, Morgane ROUXEL, OUAFAE BEAUVERGER, Mikael XU, Hongxiu SHAO, Zongze JEBBAR, Mohamed AS 1:1,2,3;2:4;3:1,2,3;4:1,2,3;5:1,2,3;6:1,2,3;7:4;8:4;9:1,2,3; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:; C1 Univ Bretagne Occidentale, IUEM, LMEE, UEB,UMR 6197, F-29280 Plouzane, France. CNRS, IUEM, LMEE, UMR 6197, F-29280 Plouzane, France. IFREMER, LMEE, UMR 6197, F-29280 Plouzane, France. SOA, Inst Oceanog 3, Key Lab Marine Biogenet Resources, Xiamen 361005, Peoples R China. C2 UBO, FRANCE CNRS, FRANCE IFREMER, LMEE, UMR 6197, F-29280 Plouzane, France. SOA, CHINA IF 2.306 TC 12 UR https://archimer.ifremer.fr/doc/00171/28204/26509.pdf LA English DT Article DE ;Thermotogales;Kosmotoga;Kosmotoga pacifica;Hydrothermal sediment AB A novel strictly anaerobic thermophilic heterotrophic bacterium, strain SLHLJ1T, was isolated from a Pacific hydrothermal sediment. Cells were Gram-negative coccobacilli (approximately 1.0 × 0.6 μm) with a toga. It grew at temperatures between 33 and 78 °C (optimum 70 °C). Elemental sulphur and l-cystine stimulated its growth. It contained C16:0, C16:1 ω11c, C18:0 and C18:1 ω9c as major fatty acids (>5 %), 3 phospholipids and 2 glycolipids as polar lipids. Its DNA G+C content was 43.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLHLJ1T within the family Thermotogaceae. The novel isolate was most closely related to Kosmotoga arenicorallina (97.93 % 16S rRNA gene sequence similarity), K. olearia (92.43 %) and K. shengliensis (92.17 %). On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons with its closest relatives, we propose its assignment to a novel species of the genus Kosmotoga. The name Kosmotoga pacifica sp. nov. is proposed with strain SLHLJ1T (=DSM 26965T = JCM 19180T = UBOCC 3254T) as the type species. PY 2014 PD JAN SO Extremophiles SN 1431-0651 PU Springer Japan Kk VL 18 IS 1 UT 000328624500008 BP 81 EP 88 DI 10.1007/s00792-013-0596-7 ID 28204 ER EF