Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10

Type Article
Date 2013-09
Language English
Author(s) Camacho-Chab Juan Carlos1, Guezennec Jean2, Chan-Bacab Manuel Jesus1, Rios-Leal Elvira3, Sinquin CorinneORCID2, Muniz-Salazar Raquel4, de La Rosa-Garcia Susana Del C.1, Reyes-Estebanez Manuela1, Ortega-Morales Benjamin Otto1
Affiliation(s) 1 : Autonomous Univ Campeche, Ctr Environm Microbiol & Biotechnol, San Francisco Campeche 24039, Campeche, Mexico.
2 : IFREMER, Lab Biotechnol & Mol Marines, F-21105 Nantes Bp, France.
3 : Natl Polytech Inst CINVESTAV, Ctr Res & Adv Studies, Dept Biotechnol & Bioengn, Mexico City 07360, DF, Mexico.
4 : Autonomous Univ Baja California, Sch Hlth Sci, Lab Mol Ecol & Epidemiol, Tijuana 22390, Baja California, Mexico.
Source International Journal Of Molecular Sciences (1422-0067) (Mdpi Ag), 2013-09 , Vol. 14 , N. 9 , P. 18959-18972
DOI 10.3390/ijms140918959
WOS© Times Cited 33
Keyword(s) bioemulsifier, emulsifying stability, non-toxic, Microbacterium sp, biotechnology
Abstract A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its highest level (94%) at 50 degrees C, pH 10 and 3.5% NaCl content. It was not toxic to Artemia salina nauplii. Microbactan is, therefore, a non-toxic and non-ionic bioemulsifier of high molecular weight with affinity for a range of oily substrates. Comparative phylogenetic assessment of the 16S rDNA gene of Microbacterium sp. MC3B-10 with genes derived from other marine Microbacterium species suggested that this genus is well represented in coastal zones. The chemical nature and stability of the bioemulsifier suggest its potential application in bioremediation of marine environments and in cosmetics.
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Camacho-Chab Juan Carlos, Guezennec Jean, Chan-Bacab Manuel Jesus, Rios-Leal Elvira, Sinquin Corinne, Muniz-Salazar Raquel, de La Rosa-Garcia Susana Del C., Reyes-Estebanez Manuela, Ortega-Morales Benjamin Otto (2013). Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10. International Journal Of Molecular Sciences, 14(9), 18959-18972. Publisher's official version : https://doi.org/10.3390/ijms140918959 , Open Access version : https://archimer.ifremer.fr/doc/00171/28260/