FN Archimer Export Format
PT J
TI Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10
BT 
AF CAMACHO-CHAB, Juan Carlos
   GUEZENNEC, Jean
   CHAN-BACAB, Manuel Jesus
   RIOS-LEAL, Elvira
   SINQUIN, Corinne
   MUNIZ-SALAZAR, Raquel
   DE LA ROSA-GARCIA, Susana del C.
   REYES-ESTEBANEZ, Manuela
   ORTEGA-MORALES, Benjamin Otto
AS 1:1;2:2;3:1;4:3;5:2;6:4;7:1;8:1;9:1;
FF 1:;2:PDG-RBE-ADM1;3:;4:;5:PDG-RBE-BRM-BMM;6:;7:;8:;9:;
C1 Autonomous Univ Campeche, Ctr Environm Microbiol & Biotechnol, San Francisco Campeche 24039, Campeche, Mexico.
   IFREMER, Lab Biotechnol & Mol Marines, F-21105 Nantes Bp, France.
   Natl Polytech Inst CINVESTAV, Ctr Res & Adv Studies, Dept Biotechnol & Bioengn, Mexico City 07360, DF, Mexico.
   Autonomous Univ Baja California, Sch Hlth Sci, Lab Mol Ecol & Epidemiol, Tijuana 22390, Baja California, Mexico.
C2 UNIV CAMPECHE, MEXICO
   IFREMER, FRANCE
   NATL POLYTECH INST CINVESTAV, MEXICO
   UNIV BAJA CALIFORNIA, MEXICO
SI BREST
   NANTES
SE PDG-RBE-ADM1
   PDG-RBE-BRM-BMM
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
   copubli-sud
IF 2.339
TC 33
UR https://archimer.ifremer.fr/doc/00171/28260/26530.pdf
LA English
DT Article
DE ;bioemulsifier;emulsifying stability;non-toxic;Microbacterium sp;biotechnology
AB A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its highest level (94%) at 50 degrees C, pH 10 and 3.5% NaCl content. It was not toxic to Artemia salina nauplii. Microbactan is, therefore, a non-toxic and non-ionic bioemulsifier of high molecular weight with affinity for a range of oily substrates. Comparative phylogenetic assessment of the 16S rDNA gene of Microbacterium sp. MC3B-10 with genes derived from other marine Microbacterium species suggested that this genus is well represented in coastal zones. The chemical nature and stability of the bioemulsifier suggest its potential application in bioremediation of marine environments and in cosmetics.
PY 2013
PD SEP
SO International Journal Of Molecular Sciences
SN 1422-0067
PU Mdpi Ag
VL 14
IS 9
UT 000328623900090
BP 18959
EP 18972
DI 10.3390/ijms140918959
ID 28260
ER

EF