FN Archimer Export Format
PT J
TI Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens
BT 
AF RENAULT, Tristan
   TCHALEU, Gwenaelle
   FAURY, Nicole
   MOREAU, Pierrick
   SEGARRA, Amelie
   BARBOSA-SOLOMIEU, Valerie
   LAPEGUE, Sylvie
AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;
FF 1:PDG-RBE-SG2M;2:PDG-RBE-AGSAE-LGP;3:PDG-RBE-SG2M-LGPMM;4:PDG-RBE-SG2M-LGPMM;5:PDG-RBE-SG2M-LGPMM;6:PDG-RBE-SG2M-LGPMM;7:PDG-RBE-SG2M-LGPMM;
C1 IFREMER, Unite Sante Genet & Microbiol Mollusques, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France.
C2 IFREMER, FRANCE
SI LA TREMBLADE
SE PDG-RBE-SG2M
   PDG-RBE-AGSAE-LGP
   PDG-RBE-SG2M-LGPMM
IN WOS Ifremer jusqu'en 2018
IF 2.815
TC 29
UR https://archimer.ifremer.fr/doc/00172/28306/26582.pdf
LA English
DT Article
AB Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order. OsHV-1 has been associated with mortality outbreaks in different bivalve species including the Pacific cupped oyster, Crassostrea gigas. Since 2008, massive mortality events have been reported among C. gigas in Europe in relation to the detection of a variant of OsHV-1, called μVar. Since 2009, this variant has been mainly detected in France. These results raise questions about the emergence and the virulence of this variant. The search for association between specific virus genetic markers and clinical symptoms is of great interest and the characterization of the genetic variability of OsHV-1 specimens is an area of growing interest. Determination of nucleotide sequences of PCR-amplified virus DNA fragments has already been used to characterize OsHV-1 specimens and virus variants have thus been described. However, the virus DNA sequencing approach is time-consuming in the high-scale format. Identification and genotyping of highly polymorphic microsatellite loci appear as a suitable approach. The main objective of the present study was the development of a genotyping method in order to characterise clinical OsHV-1 specimens by targeting a particular microsatellite locus located in the ORF4 area. Genotyping results were compared to sequences already available. An excellent correlation was found between the detected genotypes and the corresponding sequences showing that the genotyping approach allowed an accuraté discrimination between virus specimens
PY 2014
PD JAN
SO Veterinary Research
SN 0928-4249
PU Biomed Central Ltd
VL 45
IS 3
UT 000330276500001
BP 1
EP 8
DI 10.1186/1297-9716-45-3
ID 28306
ER

EF