FN Archimer Export Format
PT J
TI Plasmodium falciparum NIMA-related kinase Pfnek-1: sex specificity and assessment of essentiality for the erythrocytic asexual cycle
BT 
AF DORIN-SEMBLAT, Dominique
   SCHMITT, Sophie
   SEMBLAT, Jean-Philippe
   SICARD, Audrey
   REININGER, Luc
   GOLDRING, Dean
   PATTERSON, Shelley
   QUASHIE, Neils
   CHAKRABARTI, Debopam
   MEIJER, Laurent
   DOERIG, Christian
AS 1:1,2;2:3;3:1,2;4:1,2;5:1,2;6:4;7:5;8:;9:5;10:3;11:1,2;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;
C1 Ecole Polytech Fed Lausanne, INSERM EPFL Joint Lab, Global Hlth Inst, GHI SV EPFL Stn 19, CH-1015 Lausanne, Switzerland.
   Univ Glasgow, Wellcome Trust Ctr Mol Parasitol, INSERM U609, Glasgow G12 8TA, Lanark, Scotland.
   CNRS, Biol Stn, Cell Cycle Grp, F-29680 Roscoff, Bretagne, France.
   Univ Kwazulu Natal, Durban, South Africa.
   Univ Cent Florida, Dept Mol Biol & Microbiol, Orlando, FL 32826 USA.
C2 ECOLE POLYTECH FED LAUSANNE, SWITZERLAND
   UNIV GLASGOW, UK
   CNRS, FRANCE
   UNIV KWAZULU NATAL, SOUTH AFRICA
   UNIV CENT FLORIDA, USA
IF 3.061
TC 28
UR https://archimer.ifremer.fr/doc/00175/28578/26965.pdf
LA English
DT Article
AB The Plasmodium falciparum kinome includes a family of four protein kinases (Pfnek-1 to -4) related to the NIMA (never-in-mitosis) family, members of which play important roles in mitosis and meiosis in eukaryotic cells. Only one of these, Pfnek-1, which we previously characterized at the biochemical level, is expressed in asexual parasites. The other three (Pfnek-2, -3 and -4) are expressed predominantly in gametocytes, and a role for nek-2 and nek-4 in meiosis has been documented. Here we show by reverse genetics that Pfnek-1 is required for completion of the asexual cycle in red blood cells and that its expression in gametocytes in detectable by immunofluorescence in male (but not in female) gametocytes, in contrast with Pfnek-2 and Pfnek-4. This indicates that the function of Pfnek-1 is non-redundant with those of the other members of the Pfnek family and identifies Pfnek-1 as a potential target for antimalarial chemotherapy. A medium-throughput screen of a small-molecule library provides proof of concept that recombinant Pfnek-1 can be used as a target in drug discovery.
PY 2011
PD OCT
SO Microbiology-sgm
SN 1350-0872
PU Soc General Microbiology
VL 157
UT 000296177300005
BP 2785
EP 2794
DI 10.1099/mic.0.049023-0
ID 28578
ER

EF