FN Archimer Export Format
PT J
TI Quantification of eggs and sperm in the Black-lip pearl oyster Pinctada margaritifera using an enzyme-linked immunosorbent assay (ELISA)
BT 
AF JEUNG, Hee-Do
   KANG, Do-Hyung
   PARK, Heung-Sik
   LE MOULLAC, Gilles
   CHOI, Kwang-Sik
AS 1:1;2:2;3:2;4:3;5:1;
FF 1:;2:;3:;4:PDG-RBE-RMPF;5:;
C1 Jeju Natl Univ, Sch Marine Biomed Sci, Cheju 690756, South Korea.
   Korea Inst Ocean Sci & Technol, Global Bioresources Res Ctr, Ansan, South Korea.
   IFREMER, Ctr Pacifique, Lab Ecosyst Perlicole, Taravao, Fr Polynesia.
C2 UNIV NAT JEJU, SOUTH KOREA
   KIOST, SOUTH KOREA
   IFREMER, FRANCE
SI TAHITI
SE PDG-RBE-RMPF
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
IF 0.878
TC 4
UR https://archimer.ifremer.fr/doc/00175/28659/32145.pdf
LA English
DT Article
DE ;Black-lip pearl oyster;ELISA;Pinctada margaritifera;Polyclonal antibody;Quantification of eggs and sperm
AB We have developed immunological probes to quantify eggs and sperm of the Black-lip pearl (BLP) oyster Pinctada margaritifera. The western blot assay revealed that the polyclonal antibodies developed in this study specifically recognized only egg and sperm proteins. These polyclonal antibodies also showed high sensitivities to the antigens, detecting 0.31–10 μg/ml of the egg or sperm proteins in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, we used an indirect ELISA to quantify the eggs and sperm in BLP oysters collected in December 2009 from Weno Island in Micronesia and in May 2010 from Tahiti. The gonad somatic index (GSI), a ratio of gonad weight to somatic tissue weight, of the females collected from Weno Island ranged from 3.6 to 18.4 %, while the GSI of the females collected from Tahiti ranged from 5.6 to 12.6 %. Similarly, the GSI of the male BLP oysters from Weno Island ranged from 0.8 to 8.5 %, while the GSI of the males from Tahiti ranged from 4.8 to 7.5 %. These results lead to the conclusion that the immunological probes developed in this study can be successfully applied to quantify BLP oyster gonadal tissues and that these probes can be used in studies of BLP oyster reproductive ecology based on their sensitivity, rapidity, and affordability.
PY 2014
PD MAY
SO Fisheries Science
SN 0919-9268
PU Springer Japan Kk
VL 80
IS 3
UT 000336367100007
BP 451
EP 462
DI 10.1007/s12562-014-0706-1
ID 28659
ER

EF