FN Archimer Export Format
PT J
TI Comparative Transcriptome of Wild Type and Selected Strains of the Microalgae Tisochrysis lutea Provides Insights into the Genetic Basis, Lipid Metabolism and the Life Cycle
BT 
AF CARRIER, Gregory
   GARNIER, Matthieu
   LE CUNFF, Loic
   BOUGARAN, Gael
   PROBERT, Ian
   DE VARGAS, Colomban
   CORRE, E
   CADORET, Jean-Paul
   SAINT-JEAN, Bruno
AS 1:1;2:1;3:3;4:1;5:2;6:2;7:4;8:1;9:1;
FF 1:PDG-RBE-BRM-PBA;2:PDG-RBE-BRM-PBA;3:;4:PDG-RBE-BRM-PBA;5:;6:;7:;8:PDG-RBE-BRM-PBA;9:PDG-RBE-BRM-PBA;
C1 IFREMER PBA, Nantes, France.
   CNRS UPMC, UMR 7144, Stn Biol Roscoff, Roscoff, France.
   UMT Genovigne, Montpellier, France.
   CNRS UPMC, ABiMS, Stn Biol Roscoff, Roscoff, France.
C2 IFREMER, FRANCE
   CNRS, FRANCE
   UMT GENOVIGNE, FRANCE
   UNIV PARIS 06, FRANCE
SI NANTES
SE PDG-RBE-BRM-PBA
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.234
TC 31
UR https://archimer.ifremer.fr/doc/00175/28662/27089.pdf
LA English
DT Article
AB The applied exploitation of microalgae cultures has to date almost exclusively involved the use of wild type strains, deposited over decades in dedicated culture collections. Concomitantly, the concept of improving algae with selection programs for particular specific purposes is slowly emerging. Studying since a decade an economically and ecologically important haptophyte Tisochrysis lutea (Tiso), we took advantage of the availability of wild type (Tiso-Wt) and selected (Tiso-S2M2) strains to conduct a molecular variations study. This endeavour presented substantial challenges: the genome assembly was not yet available, the life cycle unknown and genetic diversity of Tiso-Wt poorly documented. This study brings the first molecular data in order to set up a selection strategy for that microalgae.  Following high-throughput Illumina sequencing, transcriptomes of Tiso-Wt and Tiso-S2M2 were de novo assembled and annotated. Genetic diversity between both strains was analyzed and revealed a clear conservation, while a comparison of transcriptomes allowed identification of polymorphisms resulting from the selection program. Of 34,374 transcripts, 291 were differentially expressed and 165 contained positional polymorphisms (SNP, Indel). We focused on lipid over-accumulation of the Tiso-S2M2 strain and 8 candidate genes were identified by combining analysis of positional polymorphism, differential expression levels, selection signature and by study of putative gene function. Moreover, genetic analysis also suggests the existence of a sexual cycle and genetic recombination in Tisochrysis lutea.
PY 2014
PD JAN
SO Plos One
SN 1932-6203
PU Public Library Science
VL 9
IS 1
UT 000330570000078
DI 10.1371/journal.pone.0086889
ID 28662
ER

EF