Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa

Type Article
Date 1998-01
Language English
Author(s) Suquet Marc1, Dreanno CatherineORCID1, 2, Petton Bruno1, Normant Yvon1, Omnes Marie-HeleneORCID1, Billard Roland2
Affiliation(s) 1 : IFREMER, Ctr Brest, Physiol Poissons Lab, F-29280 Plouzane, France.
2 : MNHN, France
Source Aquatic Living Resources (0990-7440) (Gauthier-villars/editions Elsevier), 1998-01 , Vol. 11 , N. 1 , P. 45-48
DOI 10.1016/S0990-7440(99)80030-8
WOS© Times Cited 43
Keyword(s) turbot, Psetta maxima, sperm, cryopreservation, hatching
Abstract The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use for routine aquaculture practices.
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