|Author(s)||Suquet Marc1, Dreanno Catherine1, 2, Petton Bruno1, Normant Yvon1, Omnes Marie-Helene1, Billard Roland2|
|Affiliation(s)||1 : IFREMER, Ctr Brest, Physiol Poissons Lab, F-29280 Plouzane, France.
2 : MNHN, France
|Source||Aquatic Living Resources (0990-7440) (Gauthier-villars/editions Elsevier), 1998-01 , Vol. 11 , N. 1 , P. 45-48|
|WOS© Times Cited||47|
|Keyword(s)||turbot, Psetta maxima, sperm, cryopreservation, hatching|
|Abstract||The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use for routine aquaculture practices.|
Suquet Marc, Dreanno Catherine, Petton Bruno, Normant Yvon, Omnes Marie-Helene, Billard Roland (1998). Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa. Aquatic Living Resources, 11(1), 45-48. Publisher's official version : https://doi.org/10.1016/S0990-7440(99)80030-8 , Open Access version : https://archimer.ifremer.fr/doc/00176/28721/