FN Archimer Export Format PT J TI Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa BT AF SUQUET, Marc DREANNO, Catherine PETTON, Bruno NORMANT, Yvon OMNES, Marie-Helene BILLARD, Roland AS 1:1;2:1,2;3:1;4:1;5:1;6:2; FF 1:;2:;3:;4:;5:;6:; C1 IFREMER, Ctr Brest, Physiol Poissons Lab, F-29280 Plouzane, France. MNHN, France C2 IFREMER, FRANCE MNHN, FRANCE SI BREST IN WOS Ifremer jusqu'en 2018 IF 0.942 TC 50 UR https://archimer.ifremer.fr/doc/00176/28721/27176.pdf LA English DT Article DE ;turbot;Psetta maxima;sperm;cryopreservation;hatching AB The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use for routine aquaculture practices. PY 1998 PD JAN SO Aquatic Living Resources SN 0990-7440 PU Gauthier-villars/editions Elsevier VL 11 IS 1 UT 000072864900006 BP 45 EP 48 DI 10.1016/S0990-7440(99)80030-8 ID 28721 ER EF