FN Archimer Export Format PT J TI Exposure to the Neurotoxic Dinoflagellate, Alexandrium catenella, Induces Apoptosis of the Hemocytes of the Oyster, Crassostrea gigas BT AF MEDHIOUB, Walid RAMONDENC, Simon VANHOVE, Audrey VERGNES, Agnes MASSERET, Estelle SAVAR, Veronique AMZIL, Zouher LAABIR, Mohamed ROLLAND, Jean-Luc AS 1:1;2:2;3:2;4:2;5:2;6:3;7:3;8:2;9:2; FF 1:;2:PDG-RBE-BOME-LALR;3:;4:PDG-RBE-BOME-LALR;5:;6:PDG-ODE-LITTORAL-PHYC;7:PDG-ODE-LITTORAL-PHYC;8:;9:PDG-RBE-BOME-LALR; C1 Inst Natl Sci & Technol Mer, Lab Milieu Marin, Salammbo 2025, Tunisia. Univ Montpellier I, Univ Montpellier 2, Inst Francais Rech Exploitat Mer, Inst Rech Dev,CNRS,UMR Ecol Syst Marins Cotiers 5, F-34095 Montpellier 5, France. Inst Francais Rech Exploitat Mer, Lab Environm Microbiol & Phycotoxines, F-44311 Nantes 3, France. C2 INSTM, TUNISIA UNIV MONTPELLIER, FRANCE IFREMER, FRANCE SI MONTPELLIER NANTES SMEL SETE SE PDG-RBE-BOME-LALR PDG-ODE-LITTORAL-PHYC IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france copubli-int-hors-europe copubli-sud IF 3.512 TC 21 UR https://archimer.ifremer.fr/doc/00176/28744/27213.pdf LA English DT Article DE ;shellfish;toxins;apoptosis;gene expression AB This study assessed the apoptotic process occurring in the hemocytes of the Pacific oyster, Crassostrea gigas, exposed to Alexandrium catenella, a paralytic shellfish toxins (PSTs) producer. Oysters were experimentally exposed during 48 h to the toxic algae. PSTs accumulation, the expression of 12 key apoptotic-related genes, as well as the variation of the number of hemocytes in apoptosis was measured at time intervals during the experiment. Results show a significant increase of the number of hemocytes in apoptosis after 29 h of exposure. Two pro-apoptotic genes (Bax and Bax-like) implicated in the mitochondrial pathway were significantly upregulated at 21 h followed by the overexpression of two caspase executor genes (caspase-3 and caspase-7) at 29 h, suggesting that the intrinsic pathway was activated. No modulation of the expression of genes implicated in the cell signaling Fas-Associated protein with Death Domain (FADD) and initiation-phase (caspase-2) was observed, suggesting that only the extrinsic pathway was not activated. Moreover, the clear time-dependent upregulation of five (Bcl2, BI-1, IAP1, IAP7B and Hsp70) inhibitors of apoptosis-related genes associated with the return to the initial number of hemocytes in apoptosis at 48 h of exposure suggests the involvement of strong regulatory mechanisms of apoptosis occurring in the hemocytes of the Pacific oyster. PY 2013 PD DEC SO Marine Drugs SN 1660-3397 PU Mdpi Ag VL 11 IS 12 UT 000330220400008 BP 4799 EP 4814 DI 10.3390/md11124799 ID 28744 ER EF