FN Archimer Export Format
PT J
TI Comparative proteomics reveals proteins impacted by nitrogen deprivation in wild-type and high lipid-accumulating mutant strains of Tisochrysis lutea
BT 
AF GARNIER, Matthieu
   CARRIER, Gregory
   ROGNIAUX, Helene
   NICOLAU, Elodie
   BOUGARAN, Gael
   SAINT-JEAN, Bruno
   CADORET, Jean-Paul
AS 1:1;2:1;3:2;4:1;5:1;6:1;7:1;
FF 1:PDG-RBE-BRM-PBA;2:PDG-RBE-BRM-PBA;3:;4:PDG-RBE-BRM-PBA;5:PDG-RBE-BRM-PBA;6:PDG-RBE-BRM-PBA;7:PDG-RBE-BRM;
C1 Ifremer, Lab BRM PBA Nantes, France.
   INRA, Biopolymers Interact Assemblies UR1268, F-44316 Nantes, France.
C2 IFREMER, FRANCE
   INRA, FRANCE
SI NANTES
SE PDG-RBE-BRM-PBA
   BIBS
   PDG-RBE-BRM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
IF 3.888
TC 39
UR https://archimer.ifremer.fr/doc/00179/29012/27470.pdf
LA English
DT Article
DE ;Microalgae;Biotechnology;Lipid;Nitrogen;Proteomic;Selection
AB Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for biomass feedstock, food and biofuel production. Using a domesticated oleaginous algae Tisochrysis lutea, we performed the first comparative proteomic analysis on the wild type strain and a selected lipid over-accumulating mutant. 2-DE analysis was made on these strains cultured in two metabolic conditions, with and without nitrogen deprivation, which revealed significant differences in proteomes according to both strain and nitrogen availability. Mass spectrometry allowed us to identify 37 proteins that were differentially expressed between the two strains, and 17 proteins regulated by nitrogen starvation concomitantly with lipid accumulation. The proteins identified are known to be involved in various metabolic pathways including lipid, carbohydrate, amino acid, energy and pigment metabolisms, photosynthesis, protein translation, stress response and cell division. Four candidates were selected for possible implication in the over-accumulation of lipids during nitrogen starvation. These include the plastid beta-ketoacyl-ACP reductase protein, the coccolith scale associated protein and two glycoside hydrolases involved in biosynthesis of fatty acids, carbon homeostasis and carbohydrate catabolism, respectively. This proteomic study confirms the impact of nitrogen starvation on overall metabolism and provides new perspectives to study the lipid over-accumulation in the prymnesiophyte haptophyte T. lutea.  Biological significance  This paper study consists of the first proteomic analysis on Tisochrysis lutea, a non-model marine microalga of interest for aquaculture and lipids production. Comparative proteomics revealed proteins putatively involved in the up-accumulation of neutral lipids in a mutant strain during nitrogen starvation. The results are of great importance for future works to improve lipid accumulation in microalgae of biotechnological interest for biofuel production. This article is part of a Special Issue entitled: Proteomics of non-model organisms.
PY 2014
PD JUL
SO Journal Of Proteomics
SN 1874-3919
PU Elsevier Science Bv
VL 105
UT 000338600000010
BP 107
EP 120
DI 10.1016/j.jprot.2014.02.022
ID 29012
ER

EF