Identification of paralytic shellfish poisons using liquid chromatography / ion mobility - high resolution mass spectrometry
|Author(s)||Poyer Salomé1, Loutelier-Bourhis Corinne1, Mondeguer Florence2, Coadou Gaël1, Hubert-Roux Marie1, Enche Julien3, Bossee Anne3, Hess Philipp2, Afonso Carlos3|
|Affiliation(s)||1 : Normandie Université, COBRA, UMR 6014 et FR 3038 ; Université de Rouen ; INSA de Rouen ; CNRS, IRCOF, 1 rue Tesnière, 76821 Mont Saint Aignan Cedex, France.
2 : IFREMER, Laboratoire Phycotoxines, F-44311 Nantes 03, France
3 : DGA Maîtrise NRBC, département Analyse Chimique, F-91710 Vert Le Petit, France
|Meeting||EuPA conference (EUropean Proteome Association) - Saint Malo (FRANCE), October 14-17, 2013|
|Abstract||Saxitoxin and its analogues also called paralytic shellfish poisons (PSPs) are very potent neurotoxins  produced by dinoflagellates and referenced as chemical weapon in the chemical warfare convention (CWC). The official detection methods for these toxins present limitations concerning their speed and reliability .
Due to the presence of isomers, not differentiable by mass spectrometry (MS), an upstream separation is necessary. In order to separate saxitoxin analogues, hydrophilic interaction liquid chromatography (HILIC) and ion mobility (IM) were used. Those techniques respectively developed for high polar compounds and three dimensional structure differentiation are particularly well adapted to the separation of PSPs. This HILIC/IM-MS coupling was used to develop a fast, reproducible and sensitive method for the separation and the detection of the PSPs