Maldi mass spectrometry imaging applied to small molecules localization

Type Poster
Date 2012-05-21
Language English
Author(s) Guitton Yann1, Mondeguer FlorenceORCID3, Nicolau ElodieORCID3, Royer Florence3, Hilbert Jean-Louis1, Fournier Isabelle2, Wisztorski Maxence2
Affiliation(s) 1 : INRA, USTL UMR SADV 1281, Stress Abiotiques et Différenciation des Végétaux Cultivés, Université Lille Nord de France, Lille 1, SN2, F-59655 Villeneuve d’Ascq, France
2 : Université Lille Nord de France, MALDI Imaging Team, Laboratoire de Spectrométrie de masse Biologique, Fondamentale, Appliquée, EA-4550, Lille 1, SN3, F-59655 Villeneuve d’Ascq, France
3 : IFREMER , Laboratoire Phycotoxines, Rue de l’ile d’Yeu, BP 21105 44311 Nantes, France
Meeting 6èmes Journées Scientifiques du Réseau Français de Métabolomique et Fluxomique (RMFM) : Echanger, partager au sein de la communauté d'acteurs de la métabolomique et de la fluxomique - 21-23 Mai 2012, Nantes
Note https://colloque4.inra.fr/6_js_reseau_francais_metabolomique_fluxomique
Abstract MALDI Mass Spectrometry Imaging (MALDI-MSI) is a two-dimensional MALDI mass spectrometric technique used to visualize the spatial distribution of a large variety of biological molecules (from small metabolites to large proteins) without extraction, purification, separation or labelling of biological samples. Since its introduction by Caprioli et al in 1997, it has become one of the most important molecular histology methods for biomarker hunting and for understanding the spatial distribution of biomolecules in various tissues. MALDI-MSI is thus ideal for complementing the expanding field of metabolomics. Despite the fact that MALDI-MSI have been very successfully applied in divers studies (even in clinical applications) its use remains far from routine, and there is still a need to adapt protocols to suit specific tissues4 or metabolites. Here we describe the methodology of MALDI-MSI with examples taken from
applications in plant (Cichorium intybus) and in seashell (Mytilus edulis).
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Guitton Yann, Mondeguer Florence, Nicolau Elodie, Royer Florence, Hilbert Jean-Louis, Fournier Isabelle, Wisztorski Maxence (2012). Maldi mass spectrometry imaging applied to small molecules localization. 6èmes Journées Scientifiques du Réseau Français de Métabolomique et Fluxomique (RMFM) : Echanger, partager au sein de la communauté d'acteurs de la métabolomique et de la fluxomique - 21-23 Mai 2012, Nantes. https://archimer.ifremer.fr/doc/00179/29041/