FN Archimer Export Format
PT J
TI Bacterial Dynamics in a Microphytobenthic Biofilm: a Tidal Mesocosm Approach
BT 
AF AGOGUE, Helene
   MALLET, Clarisse
   ORVAIN, Francis
   DE CRIGNIS, Margot
   MORNET, Francoise
   DUPUY, Christine
AS 1:1;2:2;3:3;4:1,3;5:4;6:1;
FF 1:;2:;3:;4:;5:PDG-RBE-HGS-LRHLR;6:;
C1 Univ La Rochelle, UMR CNRS Lab LIENSs 7266, F-17000 La Rochelle, France.
   Univ Clermont Ferrand, UMR CNRS 6023, Lab Microorganismes Genome & Environm, F-63177 Aubiere, France.
   Univ Caen Basse Normandie, BioMEA CNRS FRE3484, F-14032 Caen, France.
   IFREMER, Lab Ressources Halieut La Rochelle, Stn La Rochelle, F-17137 Lhoumeau, France.
C2 UNIV LA ROCHELLE, FRANCE
   UNIV CLERMONT FERRAND, FRANCE
   UNIV CAEN, FRANCE
   IFREMER, FRANCE
SI LA ROCHELLE
SE PDG-RBE-HGS-LRHLR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.99
TC 19
UR https://archimer.ifremer.fr/doc/00181/29271/27682.pdf
LA English
DT Article
DE ;Muddy sediment;Microphytobenthos;Bacteria;Extracellular polymeric substances;Interactions;Mesocosm
AB In intertidal mudflats, during low tide exposure, microphytobenthos (MPB) migrate vertically through the surface sediment and form, with the heterotrophic bacteria, a transient biofilm. Inside this biofilm, multiple interactions exist between MPB and bacteria. These micro-organisms secrete a wide range of extracellular polymeric substances (EPS), which are major components of the biofilm matrix. In this study, we used a tidal mesocosm experiment in order to decipher the interactions of the MPB-EPS-bacteria complex within the biofilm. We tried to determine if the EPS could control bacterial activities and/or production and/or richness according to the age of the biofilm and to the immersion/emersion period. The dynamics of biomasses of MPB and prokaryotes, the bacterial production, the hydrolysis of predominating organic constituents in the dissolved organic carbon (DOC) pool (i.e., carbohydrates and polypeptides), and the bacterial structure were studied in relation to the different EPS fractions (carbohydrates and proteins: colloidal and bound) dynamics during 8 days. Our experiment had emphasized the influence of the environmental conditions (light, immersion/emersion) on the interactions within the biofilm and also on the effects on biofilm ageing. Bacterial production was always inhibited by the bound EPS-carbohydrate, especially during low tide. Our results suggest that the concentration and composition of EPS had a major role in the bacterial/MPB interactions: these interactions can be either positive or negative in order to regulate the productive phases of MPB and bacteria.
PY 2014
PD SEP
SO Journal Of Sea Research
SN 1385-1101
PU Elsevier Science Bv
VL 92
UT 000342478100006
BP 36
EP 45
DI 10.1016/j.seares.2014.03.003
ID 29271
ER

EF