FN Archimer Export Format
PT J
TI Metallothionein mRNA induction is correlated with the decrease of DNA strand breaks in cadmium exposed zebra mussels
BT 
AF VINCENT-HUBERT, Francoise
   CHATEL, Amelie
   GOURLAY-FRANCE, Catherine
AS 1:1;2:1;3:1;
FF 1:;2:;3:;
C1 IRSTEA, Unite Rech Hydrosyst & Bioprocedes, F-92761 Antony, France.
C2 IRSTEA, FRANCE
IF 2.415
TC 6
UR https://archimer.ifremer.fr/doc/00184/29547/27873.pdf
LA English
DT Article
DE ;Benzo[a]pyrene;Cadmium;Metallothionein;Aryl hydrocarbon receptor;Antioxidant enzymes;RT-qPCR
AB We have previously shown that cadmium (Cd) and Benzo[a]pyrene (BaP) induced early DNA damages in zebra mussels, and that the level of DNA strand breaks (SB) returned to a basal level after 3 days of exposure to Cd. The aim of the present study was to go further in the mechanisms of Cd and BaP detoxification. For that purpose, expression of genes encoding for metallothionein (MT), Aryl Hydrocarbon Receptor (AHR), P-gP, catalase, glutathione S-transferase and Heat shock protein 70 (HSP70) proteins have been measured using RT-qPCR. Data reported here show that Cd is a strong inducer of MT and HSP70 genes, and that BaP is a strong inducer of P-gP and AHR genes. Exposure to Cd and BaP resulted in moderate changes in antioxidant enzymes mRNA. Since the increase of MT mRNA occured when the DNA SB level returned to its basal level, we can suggest that MT is implicated in cadmium detoxification.
PY 2014
PD MAY
SO Mutation Research-genetic Toxicology And Environmental Mutagenesis
SN 1383-5718
PU Elsevier Science Bv
VL 766
UT 000336701400003
BP 10
EP 15
DI 10.1016/j.mrgentox.2014.03.006
ID 29547
ER

EF