FN Archimer Export Format
PT J
TI Transcriptomic study of 39 ostreid herpesvirus 1 genes during an experimental infection
BT 
AF SEGARRA, Amelie
   FAURY, Nicole
   PEPIN, Jean-Francois
   RENAULT, Tristan
AS 1:1;2:1;3:2;4:1;
FF 1:PDG-RBE-SG2M-LGPMM;2:PDG-RBE-SG2M-LGPMM;3:PDG-ODE-LITTORAL-LERPC;4:PDG-RBE-SG2M;
C1 IFREMER, LGPMM, Unite Sante Genet & Microbiol Mollusques SG2M, F-17390 La Tremblade, France.
   IFREMER, LERPC, F-17390 La Tremblade, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
SE PDG-RBE-SG2M-LGPMM
   PDG-ODE-LITTORAL-LERPC
   PDG-RBE-SG2M
IN WOS Ifremer jusqu'en 2018
IF 2.11
TC 40
UR https://archimer.ifremer.fr/doc/00184/29549/27875.pdf
LA English
DT Article
DE ;Crassostrea gigas;OsHV-1;Viral gene expression;Inhibitors of apoptosis;Real time PCR;Elongation factor
AB Massive mortality outbreaks have been reported in France since 2008 among Pacific oysters, Crassostrea gigas, with the detection of a particular OsHV-1 variant called μVar. Virus infection can be induced in healthy spat in experimental conditions allowing to better understand the disease process, including viral gene expression.  Although gene expression of other herpesviruses has been widely studied, we provide the first study following viral gene expression of OsHV-1 over time. In this context, an in vivo transcriptomic study targeting 39 OsHV-1 genes was carried out during an experimental infection of Pacific oyster spat. For the first time, several OsHV-1 mRNAs were detected by real-time PCR at 0 h, 2 h, 4 h, 18 h, 26 h and 42 h post injection. Several transcripts were detected at 2 h post infection and at 18 h post infection for all selected ORFs. Quantification of virus gene expression at different times of infection was also carried out using an oyster housekeeping gene, Elongation factor.  Developing an OsHV-1-specific reverse transcriptase real time PCR targeting 39 viral gene appears a new tool in terms of diagnosis and can be used to complement viral DNA detection in order to monitor viral replication.
PY 2014
PD JUL
SO Journal Of Invertebrate Pathology
SN 0022-2011
PU Academic Press Inc Elsevier Science
VL 119
UT 000337216500002
BP 5
EP 11
DI 10.1016/j.jip.2014.03.002
ID 29549
ER

EF