FN Archimer Export Format
PT J
TI Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability
BT 
AF HOLDER, Thomas
   BASQUIN, Claire
   EBERT, Judith
   RANDEL, Nadine
   JOLLIVET, Didier
   CONTI, Elena
   JEKELY, Gaspar
   BONO, Fulvia
AS 1:1;2:3;3:3;4:1;5:2;6:3;7:1;8:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;
C1 Max Planck Inst Dev Biol, D-72076 Tubingen, Germany.
   UMR 7144 CNRS UPMC, Equipe GAME, Stn Biol, F-29680 Roscoff, France.
   Max Planck Inst Biochem, Dept Struct Cell Biol, D-82152 Martinsried, Germany.
C2 MAX PLANCK INST, GERMANY
   UNIV PARIS 06, FRANCE
   MAX PLANCK INST, GERMANY
IF 4.035
TC 21
UR https://archimer.ifremer.fr/doc/00185/29627/28001.pdf
LA English
DT Article
CR BIOSPEEDO
BO L'Atalante
AB Background: Alvinella pompejana is an annelid worm that inhabits deep-sea hydrothermal vent sites in the Pacific Ocean. Living at a depth of approximately 2500 meters, these worms experience extreme environmental conditions, including high temperature and pressure as well as high levels of sulfide and heavy metals. A. pompejana is one of the most thermotolerant metazoans, making this animal a subject of great interest for studies of eukaryotic thermoadaptation.  Results: In order to complement existing EST resources we performed deep sequencing of the A. pompejana transcriptome. We identified several thousand novel protein-coding transcripts, nearly doubling the sequence data for this annelid. We then performed an extensive survey of previously established prokaryotic thermoadaptation measures to search for global signals of thermoadaptation in A. pompejana in comparison with mesophilic eukaryotes. In an orthologous set of 457 proteins, we found that the best indicator of thermoadaptation was the difference in frequency of charged versus polar residues (CvP-bias), which was highest in A. pompejana. CvP-bias robustly distinguished prokaryotic thermophiles from prokaryotic mesophiles, as well as the thermophilic fungus Chaetomium thermophilum from mesophilic eukaryotes. Experimental values for thermophilic proteins supported higher CvP-bias as a measure of thermal stability when compared to their mesophilic orthologs. Proteome-wide mean CvP-bias also correlated with the body temperatures of homeothermic birds and mammals.  Conclusions: Our work extends the transcriptome resources for A. pompejana and identifies the CvP-bias as a robust and widely applicable measure of eukaryotic thermoadaptation.
PY 2013
PD JAN
SO Biology Direct
SN 1745-6150
PU Biomed Central Ltd
VL 8
IS 2
UT 000315729000002
BP 1
EP 16
DI 10.1186/1745-6150-8-2
ID 29627
ER

EF