Determination of reduced sulfur compounds by high-performance liquid chromatography in hydrothermal seawater and body fluids from Riftia pachyptila
|Author(s)||Gru Cecile1, Sarradin Pierre-Marie2, Legoff Hélène1, Narcon Sandrine1, Caprais Jean-Claude2, Lallier Francois3|
|Affiliation(s)||1 : ,Ecole Natl Super Chim Rennes, F-35700 Rennes, France.
2 : IFREMER, Brest, DRO EP, F-29280 Plouzane, France.
3 : Observ Oceanol Roscoff,UPMC, CNRS, INSU,Stn Biol,Equipe Ecophysiol, F-29682 Roscoff, France.
|Source||Analyst (0003-2654) (Royal Soc Chemistry), 1998-06 , Vol. 123 , N. 6 , P. 1289-1293|
|WOS© Times Cited||31|
|Keyword(s)||sulfide, sulfite, thiosulfate, cysteine, glutathione, hydrothermal seawater, Riftia pachyptila, biological fluids|
|Abstract||This paper describes a method for the determination of reduced sulfur compounds in hydrothermal seawater and body fluids from the hydrothermal tube worm Riftia pachyptila. Sulfur is a hey component of the hydrothermal ecosystem based on chemosynthesis, Sulfur compounds were derivatized at pH 8 (4.3 for sulfide in biological fluid) with a fluorescent reagent, monobromobimane, and separated by reverse-phase HPLC. The eluted compounds were detected by spectrofluorimetry. This method allowed: the quantitative analysis of sulfide, sulfite, thiosulfate, cysteine and glutathione in seawater, vascular blood and coelomic fluids from R, pachyptila, The detection limits were in the 0.1 mu mol l(-1) range with a precision lower than 10%, The method has been applied to hydrothermal seawater. The organisms are distributed along a gradient of sulfide (produced by the vent) and thiosulfate. Analysis of biological fluid was performed with a new sample treatment allowing the analysis of total sulfide (free and bound to haemoglobin) with results comparable to published methods.|