Determination of reduced sulfur compounds by high-performance liquid chromatography in hydrothermal seawater and body fluids from Riftia pachyptila

Type Article
Date 1998-06
Language English
Author(s) Gru Cecile1, Sarradin Pierre-MarieORCID2, Legoff Hélène1, Narcon Sandrine1, Caprais Jean-Claude2, Lallier Francois3
Affiliation(s) 1 : ,Ecole Natl Super Chim Rennes, F-35700 Rennes, France.
2 : IFREMER, Brest, DRO EP, F-29280 Plouzane, France.
3 : Observ Oceanol Roscoff,UPMC, CNRS, INSU,Stn Biol,Equipe Ecophysiol, F-29682 Roscoff, France.
Source Analyst (0003-2654) (Royal Soc Chemistry), 1998-06 , Vol. 123 , N. 6 , P. 1289-1293
DOI 10.1039/a800032h
WOS© Times Cited 29
Keyword(s) sulfide, sulfite, thiosulfate, cysteine, glutathione, hydrothermal seawater, Riftia pachyptila, biological fluids
Abstract This paper describes a method for the determination of reduced sulfur compounds in hydrothermal seawater and body fluids from the hydrothermal tube worm Riftia pachyptila. Sulfur is a hey component of the hydrothermal ecosystem based on chemosynthesis, Sulfur compounds were derivatized at pH 8 (4.3 for sulfide in biological fluid) with a fluorescent reagent, monobromobimane, and separated by reverse-phase HPLC. The eluted compounds were detected by spectrofluorimetry. This method allowed: the quantitative analysis of sulfide, sulfite, thiosulfate, cysteine and glutathione in seawater, vascular blood and coelomic fluids from R, pachyptila, The detection limits were in the 0.1 mu mol l(-1) range with a precision lower than 10%, The method has been applied to hydrothermal seawater. The organisms are distributed along a gradient of sulfide (produced by the vent) and thiosulfate. Analysis of biological fluid was performed with a new sample treatment allowing the analysis of total sulfide (free and bound to haemoglobin) with results comparable to published methods.
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Gru Cecile, Sarradin Pierre-Marie, Legoff Hélène, Narcon Sandrine, Caprais Jean-Claude, Lallier Francois (1998). Determination of reduced sulfur compounds by high-performance liquid chromatography in hydrothermal seawater and body fluids from Riftia pachyptila. Analyst, 123(6), 1289-1293. Publisher's official version : https://doi.org/10.1039/a800032h , Open Access version : https://archimer.ifremer.fr/doc/00186/29763/