FN Archimer Export Format
PT J
TI Determination of reduced sulfur compounds by high-performance liquid chromatography in hydrothermal seawater and body fluids from Riftia pachyptila
BT 
AF GRU, Cecile
   SARRADIN, Pierre-Marie
   LEGOFF, Hélène
   NARCON, Sandrine
   CAPRAIS, Jean-Claude
   LALLIER, Francois
AS 1:1;2:2;3:1;4:1;5:2;6:3;
FF 1:;2:;3:;4:;5:;6:;
C1 ,Ecole Natl Super Chim Rennes, F-35700 Rennes, France.
   IFREMER, Brest, DRO EP, F-29280 Plouzane, France.
   Observ Oceanol Roscoff,UPMC, CNRS, INSU,Stn Biol,Equipe Ecophysiol, F-29682 Roscoff, France.
C2 ENSCR, FRANCE
   IFREMER, FRANCE
   OBSERV OCEANOL ROSCOFF, FRANCE
SI BREST
IN WOS Ifremer jusqu'en 2018
IF 1.818
TC 34
UR https://archimer.ifremer.fr/doc/00186/29763/28203.pdf
LA English
DT Article
CR HERO91
   HOT-96
BO Le Nadir
DE ;sulfide;sulfite;thiosulfate;cysteine;glutathione;hydrothermal seawater;Riftia pachyptila;biological fluids
AB This paper describes a method for the determination of reduced sulfur compounds in hydrothermal seawater and body fluids from the hydrothermal tube worm Riftia pachyptila. Sulfur is a hey component of the hydrothermal ecosystem based on chemosynthesis, Sulfur compounds were derivatized at pH 8 (4.3 for sulfide in biological fluid) with a fluorescent reagent, monobromobimane, and separated by reverse-phase HPLC. The eluted compounds were detected by spectrofluorimetry. This method allowed: the quantitative analysis of sulfide, sulfite, thiosulfate, cysteine and glutathione in seawater, vascular blood and coelomic fluids from R, pachyptila, The detection limits were in the 0.1 mu mol l(-1) range with a precision lower than 10%, The method has been applied to hydrothermal seawater. The organisms are distributed along a gradient of sulfide (produced by the vent) and thiosulfate. Analysis of biological fluid was performed with a new sample treatment allowing the analysis of total sulfide (free and bound to haemoglobin) with results comparable to published methods.
PY 1998
PD JUL
SO Analyst
SN 0003-2654
PU Royal Soc Chemistry
VL 123
IS 6
UT 000074256300023
BP 1289
EP 1293
DI 10.1039/a800032h
ID 29763
ER

EF