FN Archimer Export Format
PT J
TI Development of a reference artificial sediment for chemical testing adapted to the MELA sediment contact assay
BT 
AF LE BIHANIC, Florane
   PERRICHON, Prescilla
   LANDI, Laure
   CLERANDEAU, Christelle
   LE MENACH, Karyn
   BUDZINSKI, Helene
   COUSIN, Xavier
   CACHOT, Jerome
AS 1:1;2:2;3:1;4:1;5:1;6:1;7:2,3;8:1;
FF 1:;2:PDG-RBE-BE;3:;4:;5:;6:;7:;8:PDG-DEL-PC;
C1 Univ Bordeaux, EPOC UMR CNRS 5805, F-33405 Talence, France.
   IFREMER, Ecotoxicol Lab, F-17137 Lhoumeau, France.
   INRA LPGP, F-35042 Rennes, France.
C2 UNIV BORDEAUX, FRANCE
   IFREMER, FRANCE
   INRA, FRANCE
SI LA ROCHELLE
   NANTES
SE PDG-RBE-BE
   PDG-RBE-BE-LBEX
   PDG-DEL-PC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
IF 2.828
TC 12
UR https://archimer.ifremer.fr/doc/00187/29872/28479.pdf
LA English
DT Article
DE ;Artificial sediment;Spiked sediment;PAHs;Japanese medaka embryos;Embryotoxicity;Teratogenicity
AB Most persistent organic pollutants, due to their hydrophobic properties, accumulate in aquatic sediments and represent a high risk for sediment quality. To assess the toxicity of hydrophobic pollutants, a novel approach was recently proposed as an alternative to replace, refine and reduce animal experimentation: the medaka embryo–larval sediment contact assay (MELAc). This assay is performed with Japanese medaka embryos incubated on a natural sediment spiked with the compound being tested. With the aim of improving this assay, our study developed a reference exposure protocol with an artificial sediment specifically designed to limit natural sediment composition uncertainties and preparation variability. The optimum composition of the new artificial sediment was tested using a model polycyclic aromatic hydrocarbon (PAH), fluoranthene. The sediment was then validated with two other model PAHs, benz[a]anthracene and benzo[a]pyrene. Various developmental end points were recorded, including survival, embryonic heartbeat, hatching delay, hatching success, larval biometry and abnormalities. The final artificial sediment composition was set at 2.5 % dry weight (dw) Sphagnum peat, 5 % dw kaolin clay and 92.5 % dw silica of 0.2- to 0.5-mm grain size. In contrast with natural sediments, the chemical components of this artificial matrix are fully defined and readily identifiable. It is totally safe for fish embryos and presents relatively high sorption capacities for hydrophobic compounds. Studies with other hydrophobic and metallic contaminants and mixtures should be performed to further validate this artificial sedimen
PY 2014
PD DEC
SO Environmental Science And Pollution Research
SN 0944-1344
PU Springer Heidelberg
VL 21
IS 24
UT 000345314500002
BP 13689
EP 13702
DI 10.1007/s11356-014-2607-3
ID 29872
ER

EF