Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish—part II: behavior

Type Article
Date 2014-12
Language English
Author(s) Vignet Caroline1, Le Menach Karyn2, Lyphout Laura1, 3, Guionnet Tiphaine1, Frere Laura3, Leguay Didier3, Budzinski Helene2, Cousin XavierORCID1, 4, Begout Marie-LaureORCID3
Affiliation(s) 1 : IFREMER, Lab Ecotoxicol, F-17137 Lhoumeau, France.
2 : Univ Bordeaux 1, EPOC, UMR CNRS 5805, F-33405 Talence, France.
3 : IFREMER, Lab Ressources Halieut, F-17137 Lhoumeau, France.
4 : INRA LPGP, F-35042 Rennes, France.
Source Environmental Science And Pollution Research (0944-1344) (Springer Heidelberg), 2014-12 , Vol. 21 , N. 24 , P. 13818-13832
DOI 10.1007/s11356-014-2762-6
WOS© Times Cited 6
Keyword(s) Danio rerio, Polycyclic aromatic hydrocarbon, Heavy oil, Light crude oil, Anxiety-like behavior, Photomotor responses, Maze, Novel tank
Abstract In the last 10 years, behavior assessment has been developed as an indicator of neurotoxicity and an integrated indicator of physiological disruption. Polycyclic aromatic hydrocarbon (PAH) release into the environment has increased in recent decades resulting in high concentrations of these compounds in the sediment of contaminated areas. We evaluated the behavioral consequences of long-term chronic exposure to PAHs, by exposing zebrafish to diets spiked with three PAH fractions at environmentally relevant concentrations. Fish were exposed to these chemicals from their first meal (5 days postfertilization) until they became reproducing adults (at 6 months old). The fractions used were representative of PAHs of pyrolytic (PY) origin and of two oils differing in composition (a heavy fuel oil (HO) and a light crude oil (LO)). Several tests were carried out to evaluate circadian spontaneous swimming activity, responses to a challenge (photomotor response), exploratory tendencies, and anxiety levels. We found that dietary PAH exposure was associated with greater mobility, lower levels of exploratory activity, and higher levels of anxiety, particularly in fish exposed to the HO fraction and, to a lesser extent, the LO fraction. Finally, our results indicate that PAH mixtures of different compositions, representative of situations encountered in the wild, can induce behavioral disruptions resulting in poorer fish performance.
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Vignet Caroline, Le Menach Karyn, Lyphout Laura, Guionnet Tiphaine, Frere Laura, Leguay Didier, Budzinski Helene, Cousin Xavier, Begout Marie-Laure (2014). Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish—part II: behavior. Environmental Science And Pollution Research, 21(24), 13818-13832. Publisher's official version : https://doi.org/10.1007/s11356-014-2762-6 , Open Access version : https://archimer.ifremer.fr/doc/00188/29910/