FN Archimer Export Format
PT J
TI Responses of the European flounder (Platichthys flesus) to a mixture of PAHs and PCBs in experimental conditions
BT 
AF DUPUY, Celie
   GALLAND, Claire
   DEVAUX, Alain
   BONY, Sylvie
   LOIZEAU, Veronique
   DANION, Morgane
   PICHEREAU, Vianney
   FOURNIER, Michel
   LAROCHE, Jean
AS 1:1,2;2:1;3:3,4;4:3,4;5:5;6:6;7:1;8:2;9:1;
FF 1:;2:;3:;4:;5:PDG-RBE-BE-LBCO;6:;7:;8:;9:;
C1 Univ Bretagne Occidentale, Univ Europeenne Bretagne, Inst Univ Europeen Mer, UMR CNRS UBO IRD Ifremer 6523,Lab Sci Environm Ma, F-29280 Plouzane, France.
   Inst Natl Rech Sci, Inst Armand Frappier, Laval, PQ H7V 1B7, Canada.
   Univ Lyon, UMR LEHNA CNRS 5023, F-69100 Villeurbanne, France.
   INRA, USC IGH 1369, ENTPE, F-69518 Vaulx En Velin, France.
   IFREMER, Ctr Brest, Unite Biogeochim & Ecotoxicol, F-29280 Plouzane, France.
   ANSES, Agence Natl Securite Ssanit Alimentat Environm &, Lab Ploufragan Plouzane, F-29280 Plouzane, France.
C2 UBO, FRANCE
   INRS IAF, CANADA
   UNIV LYON, FRANCE
   INRA, FRANCE
   IFREMER, FRANCE
   ANSES, FRANCE
SI BREST
SE ANSES
   PDG-RBE-BE-LBCO
   UBOLEMAR
   UBO
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
   copubli-int-hors-europe
IF 2.828
TC 13
UR https://archimer.ifremer.fr/doc/00188/29914/28374.pdf
LA English
DT Article
DE ;Mixture of contaminants;Fish;Biomarker;Immunotoxicity;Detoxification process;DNA damage;PAHs;PCBs
AB A multibiomarker approach was developed to evaluate the juvenile European flounder responses to a complex mixture of 9 polycyclic aromatic hydrocarbons (PAHs) and 12 polychlorinated biphenyls (PCBs). Exposure was performed through contaminated food pellets displaying: (1) PAH and PCB levels similar to those detected in the heavily polluted Seine estuary, respectively in sediments and in flatfish and (2) ten times these concentrations. Several biomarkers of the immune system (e.g., lysozyme concentration and gene expression of complement component C3 and TNF-receptor), DNA damage (e.g., Comet assay), energetic metabolism (e.g., activity of cytochrome C oxidase), detoxification process (e.g., cytochrome P450 1A1 expression level: CYP1A1; betaine homocysteine methyl transferase expression level: BHMT) were investigated after 14 and 29 days of contamination, followed by a 14-days recovery period. After 29 days of contamination, the detoxification activity (CYP1A1 expression level) was positively correlated with DNA damages; the increase of the BHMT expression level could also be related to the detoxification process. Furthermore, after the recovery period, some biomarkers were still upregulated (i.e., CYP1A1 and BHMT expression levels). The immune system was significantly modulated by the chemical stress at the two concentration levels, and the lysozyme appeared to be the most sensitive marker of the mixture impact.
PY 2014
PD DEC
SO Environmental Science And Pollution Research
SN 0944-1344
PU Springer Heidelberg
VL 21
IS 24
UT 000345314500010
BP 13789
EP 13803
DI 10.1007/s11356-014-2563-y
ID 29914
ER

EF