FN Archimer Export Format
PT J
TI A Microarray-Based Analysis of Gametogenesis in Two Portuguese Populations of the European Clam Ruditapes decussatus
BT 
AF SOUSA, Joana
   MILAN, Massimo
   BARGELLONI, Luca
   PAULETTO, Marianna
   MATIAS, Domitilia
   JOAQUIM, Sandra
   MATIAS, Ana Margarete
   QUILLIEN, Virgile
   LEITAO, Alexandra
   HUVET, Arnaud
AS 1:1,2;2:3;3:3;4:3;5:2;6:2;7:2;8:1;9:2;10:1;
FF 1:PDG-RBE-PFOM-PI;2:;3:;4:;5:;6:;7:;8:PDG-RBE-PFOM-PI;9:;10:PDG-RBE-PFOM-PI;
C1 UMR CNRS 6539, IFREMER, Lab Sci Environm Marin, Plouzane, France.
   IPMA, Olhao, Portugal.
   Univ Padua, Dept Comparat Biomed & Food Sci, Legnaro, Italy.
C2 IFREMER, FRANCE
   IPMA, PORTUGAL
   UNIV PADUA, ITALY
SI BREST
SE PDG-RBE-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 3.234
TC 11
UR https://archimer.ifremer.fr/doc/00188/29935/28623.pdf
LA English
DT Article
AB The European clam, Ruditapes decussatus is a species with a high commercial importance in Portugal and other Southern European countries. Its production is almost exclusively based on natural recruitment, which is subject to high annual fluctuations. Increased knowledge of the natural reproductive cycle of R. decussatus and its molecular mechanisms would be particularly important in providing new highly valuable genomic information for better understanding the regulation of reproduction in this economically important aquaculture species. In this study, the transcriptomic bases of R. decussatus reproduction have been analysed using a custom oligonucleotide microarray representing 51,678 assembled contigs. Microarray analyses were performed in four gonadal maturation stages from two different Portuguese wild populations, characterized by different responses to spawning induction when used as progenitors in hatchery. A comparison between the two populations elucidated a specific pathway involved in the recognition signals and binding between the oocyte and components of the sperm plasma membrane. We suggest that this pathway can explain part of the differences in terms of spawning induction success between the two populations. In addition, sexes and reproductive stages were compared and a correlation between mRNA levels and gonadal area was investigated. The lists of differentially expressed genes revealed that sex explains most of the variance in gonadal gene expression. Additionally, genes like Foxl2, vitellogenin, condensing 2, mitotic apparatus protein p62, Cep57, sperm associated antigens 6, 16 and 17, motile sperm domain containing protein 2, sperm surface protein Sp17, sperm flagellar proteins 1 and 2 and dpy-30, were identified as being correlated with the gonad area and therefore supposedly with the number and/or the size of the gametes produced.
PY 2014
PD MAR
SO Plos One
SN 1932-6203
PU Public Library Science
VL 9
IS 3
UT 000333259900102
DI 10.1371/journal.pone.0092202
ID 29935
ER

EF