FN Archimer Export Format PT J TI Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes BT AF SEESAO, Y. AUDEBERT, C. VERREZ-BAGNIS, Veronique MERLIN, S. JEROME, Marc VISCOGLIOSI, E. DEI-CAS, E. ALIOUAT-DENIS, C. M. GAY, Melanie AS 1:1,2;2:3,5;3:4;4:3,5;5:4;6:2;7:2;8:2;9:1; FF 1:;2:;3:PDG-RBE-BRM-LEMMMB;4:;5:PDG-RBE-BRM-LEMMMB;6:;7:;8:;9:; C1 ANSES, Lab Securite Aliments, Dept Prod Peche & Aquaculture, Boulogne Sur Mer, France. Univ Lille Nord France, Inst Pasteur Lille, Ctr Infect & Immunite Lille, UMR CNRS 8204,Inserm U1019, F-59019 Lille, France. Genes Diffus, Douai, France. IFREMER, Nantes, France. Inst Pasteur, PEGASE Biosci, Lille, France. C2 ANSES, FRANCE UNIV LILLE, FRANCE GENES DIFFUSION, FRANCE IFREMER, FRANCE INST PASTEUR, FRANCE SI NANTES SE PDG-RBE-BRM-LEMMMB IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.026 TC 6 UR https://archimer.ifremer.fr/doc/00189/30020/28607.pdf LA English DT Article DE ;Anisakidae;High-throughput sequencing;Nematode;Pooled DNA extraction;Marine fish;COX2 AB Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions. PY 2014 PD JUN SO Journal Of Microbiological Methods SN 0167-7012 PU Elsevier Science Bv VL 102 UT 000337857800013 BP 69 EP 72 DI 10.1016/j.mimet.2014.05.004 ID 30020 ER EF