FN Archimer Export Format
PT J
TI Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure
BT 
AF LASSUDRIE, Malwenn
   SOUDANT, Philippe
   RICHARD, Gaelle
   HENRY, Nicolas
   MEDHIOUB, Walid
   DA SILVA, Patricia Mirella
   DONVAL, Anne
   BUNEL, Melanie
   LE GOIC, Nelly
   LAMBERT, Christophe
   DE MONTAUDOUIN, Xavier
   FABIOUX, Caroline
   HEGARET, Helene
AS 1:1;2:1;3:1;4:1;5:1,2,3;6:4;7:1;8:1;9:1;10:1;11:5;12:1;13:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;
C1 UBO CNRS, Inst Univ Europeen Mer, Lab Sci Environm Marin LEMAR, F-29280 Plouzane, France.
   IFREMER, Lab Phycotoxines, F-44311 Nantes 3, France.
   Inst Natl Sci & Technol Mer, Lab Milieu Marin, Salammbo 2025, Tunisia.
   Univ Fed Paraiba, Exact & Nat Sci Ctr, Dept Mol Biol, Lab Immunol & Pathol Invertebrates, BR-58051900 Joao Pessoa, PB, Brazil.
   Univ Bordeaux UMR 5805 EPOC, Stn Marine Arcachon, F-33120 Arcachon, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
   INSTM, TUNISIA
   UNIV FED PARAIBA, BRAZIL
   UNIV BORDEAUX, FRANCE
SI NANTES
SE PDG-DOP-DCN-EMP-PHYC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 3.451
TC 33
UR https://archimer.ifremer.fr/doc/00189/30022/28507.pdf
   https://archimer.ifremer.fr/doc/00189/30022/28814.pdf
LA English
DT Article
BO Planula IV
DE ;Bivalve;Harmful algal bloom;Oxidative stress;Venerupis philippinarum;Alexandrium ostenfeldii;Perkinsus olseni
AB Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the infection (“Light-Moderate” and “Moderate-Heavy”). Hemocyte and plasma responses, digestive enzyme activities, antioxidant enzyme activities in gills, and histopathological responses were analyzed. Reactive oxygen species (ROS) production in hemocytes and catalase (CAT) activity in gills increased with P. olseni intensity of infection in control clams fed T-Iso, but did not vary among A. ostenfeldii-exposed clams. Exposure to A. ostenfeldii caused tissue alterations associated with an inflammatory response and modifications in hemocyte morphology. In the gills, superoxide dismutase (SOD) activity decreased, and an increase in brown cell occurrence was seen, suggesting oxidative stress. Observations of hemocytes and brown cells in tissues during exposure and depuration suggest involvement of both cell types in detoxication processes. Results suggest that exposure to A. ostenfeldii disrupted the pro-/anti-oxidant response of clams to heavy P. olseni intensity. In addition, depressed mitochondrial membrane potential (MMP) in hemocytes of clams exposed to A. ostenfeldii suggests that mitochondrial functions are regulated to maintain homeostasis of digestive enzyme activity and condition index.
PY 2014
PD SEP
SO Aquatic Toxicology
SN 0166-445X
PU Elsevier Science Bv
VL 154
UT 000340339800004
BP 27
EP 38
DI 10.1016/j.aquatox.2014.05.002
ID 30022
ER

EF