FN Archimer Export Format
PT J
TI First description of French V. tubiashii strains pathogenic to mollusk: I. Characterization of isolates and detection during mortality events
BT 
AF TRAVERS, Marie-Agnes
   MERSNI ACHOUR, Rachida
   HAFFNER, Philippe
   TOURBIEZ, Delphine
   CASSONE, Anne-Laure
   MORGA, Benjamin
   DOGHRI, Ibtissem
   GARCIA, Celine
   RENAULT, Tristan
   FRUITIER-ARNAUDIN, Ingrid
   SAULNIER, Denis
AS 1:1;2:1,2,3;3:1;4:1;5:1;6:1;7:2;8:1;9:1;10:2,3;11:1;
FF 1:PDG-RBE-SG2M-LGPMM;2:;3:PDG-RBE-SG2M-LGPMM;4:PDG-RBE-SG2M-LGPMM;5:;6:PDG-RBE-SG2M-LGPMM;7:;8:PDG-RBE-SG2M-LGPMM;9:PDG-RBE-SG2M;10:;11:PDG-RBE-RMPF;
C1 IFREMER, LGPMM, SG2M, F-17390 La Tremblade, France.
   Univ La Rochelle, CNRS, LIENSs, Equipe Approches Mol,UMR 7266, F-17042 La Rochelle, France.
   Univ La Rochelle, Fédération de Recherche en Environnement et Développement Durable, FR CNRS 3097, F-17042 La Rochelle, France.
C2 IFREMER, FRANCE
   CNRS, FRANCE
   UNIV LA ROCHELLE, FRANCE
SI LA TREMBLADE
   BREST
   TAHITI
SE PDG-RBE-SG2M-LGPMM
   PDG-RBE-PFOM-PI
   PDG-RBE-SG2M
   PDG-RBE-RMPF
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.11
TC 28
UR https://archimer.ifremer.fr/doc/00189/30028/28743.pdf
LA English
DT Article
DE ;Crassostrea gigas;Pathogenicity;Extracellular products;Polyphasic approach, Real time PCR
AB Nine dominant bacterial isolates were obtained from different batches of Crassostrea gigas spat experiencing high mortality rates in a French experimental hatchery/nursery in 2007. Using phenotypic analysis combined with multilocus sequence analysis, the isolates were shown to be genetically close to the Vibrio tubiashii type strain. Based on (1) analyses of the recA gene sequences; (2) the results of DNA–DNA hybridization assays between 07/118 T2 (LMG 27884 = CECT 8426), which is a representative strain, and the V. tubiashii type strain (69%); and (3) phenotypic traits, the bacteria were classified in a group close to American V. tubiashii strain. Its virulence (70% of mortalities) and the toxicity of the extracellular products of 07/118 T2 was demonstrated (41% of mortalities). Moreover, a QPCR diagnostic tool targeting the gyrB gene was developed to investigate the epidemiological significance of V. tubiashii in French oyster mortality outbreaks recorded by the national surveillance network. Of the 21 batches originating from hatcheries, only two were positive, whereas V. tubiashii DNA could not be detected in any of the batches of moribund animals collected in field/outdoor facilities. These results demonstrate the existence of a group of virulent V. tubiashii in France that episodically infect C. gigas.
PY 2014
PD NOV
SO Journal Of Invertebrate Pathology
SN 0022-2011
PU Academic Press Inc Elsevier Science
VL 123
UT 000344724600007
BP 38
EP 48
DI 10.1016/j.jip.2014.04.009
ID 30028
ER

EF