FN Archimer Export Format
PT J
TI Survival, Growth and Reproduction of Cryopreserved Larvae from a Marine Invertebrate, the Pacific Oyster (Crassostrea gigas)
BT 
AF SUQUET, Marc
   LABBE, Catherine
   PUYO, Sophie
   MINGANT, Christian
   QUITTET, Benjamin
   BOULAIS, Myrina
   QUEAU, Isabelle
   RATISKOL, Dominique
   DISS, Blandine
   HAFFRAY, Pierrick
AS 1:1;2:2;3:3;4:1;5:3;6:1;7:1;8:1;9:4;10:3;
FF 1:PDG-RBE-PFOM-PI;2:;3:;4:PDG-RBE-PFOM-PI;5:;6:PDG-RBE-PFOM-PI;7:PDG-RBE-PFOM-PI;8:PDG-RBE-PFOM-PI;9:;10:;
C1 IFREMER, PFOM Dept, Stn Expt Argenton, UMR 6539, Argenton, France.
   INRA, LPGP, UR 1037, Rennes, France.
   SYSAAF, LPGP, Rennes, France.
   Satmar, Barfleur, France.
C2 IFREMER, FRANCE
   INRA, FRANCE
   SYSAAF, FRANCE
   SATMAR, FRANCE
SI ARGENTON
   BREST
SE PDG-RBE-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
IF 3.234
TC 20
UR https://archimer.ifremer.fr/doc/00190/30092/28635.pdf
LA English
DT Article
AB This study is the first demonstration of successful post-thawing development to reproduction stage of diploid cryopreserved larvae in an aquatic invertebrate. Survival, growth and reproductive performances were studied in juvenile and adult Pacific oysters grown from cryopreserved embryos. Cryopreservation was performed at three early stages: trochophore (13 +/- 2 hours post fertilization: hpf), early D-larvae (24 +/- 2 hpf) and late D-larvae (43 +/- 2 hpf). From the beginning (88 days) at the end of the ongrowing phase (195 days), no mortality was recorded and mean body weights did not differ between the thawed oysters and the control. At the end of the growing-out phase (982 days), survival of the oysters cryopreserved at 13 +/- 2 hpf and at 43 +/- 2 hpf was significantly higher (P<0.001) than those of the control (non cryopreserved larvae). Only the batches cryopreserved at 24 +/- 2 hpf showed lower survival than the control. Reproductive integrity of the mature oysters, formely cryopreserved at 13 +/- 2 hpf and 24 +/- 2 hpf, was estimated by the sperm movement and the larval development of their offspring in 13 crosses gamete pools (five males and five females in each pool). In all but two crosses out of 13 tested (P<0.001), development rates of the offspring were not significantly different between frozen and unfrozen parents. In all, the growth and reproductive performances of oysters formerly cryopreserved at larval stages are close to those of controls. Furthermore, these performances did not differ between the three initial larval stages of cryopreservation. The utility of larvae cryopreservation is discussed and compared with the cryopreservation of gametes as a technique for selection programs and shellfish cryobanking.
PY 2014
PD APR
SO Plos One
SN 1932-6203
PU Public Library Science
VL 9
IS 4
UT 000334103000079
DI 10.1371/journal.pone.0093486
ID 30092
ER

EF