FN Archimer Export Format PT J TI Selection and characterization of potential probiotic bacteria for Litopenaeus stylirostris shrimp hatcheries in New Caledonia BT AF PHAM, Dominique ANSQUER, Dominique CHEVALIER, Anne DAUGA, Clement PEYRAMALE, Aude WABETE, Nelly LABREUCHE, Yannick AS 1:1;2:1;3:1;4:1;5:1;6:1;7:2,3,4; FF 1:PDG-RBE-LEADNC;2:PDG-RBE-LEADNC;3:PDG-RBE-LEADNC;4:PDG-RBE-LEADNC;5:PDG-RBE-LEADNC-LAC;6:PDG-RBE-LEADNC;7:PDG-RBE-PFOM; C1 IFREMER, Dept Lagons Ecosyst & Aquaculture Durables Nouvel, Noumea 98846, New Caledonia. IFREMER, Dept Physiol Fonct Organismes Marins, Ctr Bretagne ZI Pointe du Diable, F-29280 Plouzane, France. Univ Paris 06, Univ Paris 04, UMR 8227, Stn Biol Roscoff, F-29688 Roscoff, France. CNRS, UMR 8227, Stn Biol Roscoff, F-29688 Roscoff, France. C2 IFREMER, FRANCE IFREMER, FRANCE UNIV PARIS 06, FRANCE CNRS, FRANCE SI SAINT VINCENT NOUMEA ROSCOFF SE PDG-RBE-LEADNC PDG-RBE-LEADNC-LAC PDG-RBE-PFOM IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 1.878 TC 19 UR https://archimer.ifremer.fr/doc/00190/30129/28591.pdf LA English DT Article DE ;shrimp;Litopenaeus stylirostris;probiotic;hatchery;Pseudoalteromonas;immune response AB In New Caledonia, shrimp hatcheries are confronted with mass mortality in the larval stages, a phenomenon poorly understood as no specific causative agent has been identified. This has resulted in an excessive use of prophylactic antibiotics, although their adverse effects in aquaculture are notorious. The present work was thus aimed at selecting potential probiotic strains for penaeid hatcheries. From a pool of more than 400 marine bacterial isolates sampled from the local marine environment seven strains exhibited in vitro antagonistic activity towards Vibrio harveyi. These isolates were characterized both phenotypically and genotypically using a biochemical approach and 16S rDNA sequencing. Six out of these seven strains were found to belong to the genus Pseudoalteromonas, the last one belonging to the Vibrionaceae family and related to the Harveyi clade. Selected probiotic candidates were individually tested for antagonistic activities in vitro using a green fluorescent protein (GFP)-labelled transconjugant of V. harveyi and for inherent pathogenicity towards cultured shrimp larvae at two different developmental stages. This approach enabled rapid processing and selection of candidates to be tested in our experimental hatchery. Four different experiments were conducted to test candidate strains either alone or in combination. Repeated trials showed that postlarval survival was significantly improved by adding the strain NC201 individually to the rearing water compared to unchallenged controls. The analysis of immune-related gene expressions showed that the Litsty PEN3 transcript abundance of larvae was significantly increased after being reared in probiotic-containing water; however no significant difference in lysozyme gene expression was recorded in this study. Together, these results open new insights into the use of these strains as potential substitutes to antibiotherapy in shrimp larval rearing in New Caledonia. PY 2014 PD AUG SO Aquaculture SN 0044-8486 PU Elsevier Science Bv VL 432 UT 000342247200060 BP 475 EP 482 DI 10.1016/j.aquaculture.2014.04.031 ID 30129 ER EF