FN Archimer Export Format PT J TI Effects of water accommodated fractions of crude oils and diesel on a suite of biomarkers in Atlantic cod (Gadus morhua) BT AF HOLTH, T. F. EIDSVOLL, D. P. FARMEN, E. SANDERS, M. B. MARTINEZ-GOMEZ, C. BUDZINSKI, H. BURGEOT, Thierry GUILHERMINO, L. HYLLAND, K. AS 1:1;2:1;3:2;4:3;5:4;6:5;7:6;8:7,8;9:1; FF 1:;2:;3:;4:;5:;6:;7:PDG-RBE-BE;8:;9:; C1 Univ Oslo, Dept Biosci, N-0316 Oslo, Norway. Norwegian Inst Water Res NIVA, N-0349 Oslo, Norway. CEFAS Weymouth Lab, Weymouth DT4 8UB, Dorset, England. Inst Espanol Oceanog, San Pedro Del Pinatar 30740, Spain. Univ Bordeaux, EPOC LPTC UMR CNRS 5805, Talence, France. IFREMER, Unit Res Biogeochem & Ecotoxicol, F-44311 Nantes, France. Univ Porto, CIIMAR Interdisciplinary Ctr Marine & Environm Re, Lab Ecotoxicol & Ecol, P-4050123 Oporto, Portugal. Univ Porto, ICBAS Inst Biomed Sci Abel Salazar, Dept Populat Studies, Lab Ecotoxicol, P-4050123 Oporto, Portugal. C2 UNIV OSLO, NORWAY NIVA, NORWAY CEFAS, UK IEO, SPAIN UNIV BORDEAUX, FRANCE IFREMER, FRANCE UNIV PORTO, PORTUGAL UNIV PORTO, PORTUGAL SI NANTES SE PDG-RBE-BE IN WOS Ifremer jusqu'en 2018 copubli-france copubli-europe copubli-univ-france IF 3.451 TC 43 UR https://archimer.ifremer.fr/doc/00190/30131/28593.pdf LA English DT Article DE ;Atlantic cod;Biomarkers;Weathered crude oil;Ship-diesel;Water accommodated fraction;Time-course AB The aim of this study was to characterise concentration- and time-dependent responses in juvenile Atlantic cod (Gadus morhua) following exposure for one and three weeks to the water-soluble fraction (WAF) of three weathered oils: Arabian Light crude oil (ALC), North Sea crude oil (NSC) and ship-diesel. The sum of polycyclic aromatic hydrocarbons (PAH) in water was highest after one week of exposure and within environmentally relevant concentrations. PAH metabolites in bile confirmed exposure to and uptake of PAHs. Hepatic cytochrome P450 1A (CYP1A) gene expression (here and later the phrase gene expression is used synonymously to gene transcription, although it is acknowledged that gene expression is also regulated by, e.g., translation and protein stability) increased dramatically following exposure to all three oil types (fold-change up to 165), and there was a time lag between gene and protein expression. Hepatic CYP1A protein concentration and ethoxyresorufin-O-deethylase (EROD) activity were however more variable among individuals and treatments than gene expression. Hepatic and gill EROD increased in fish exposed to WAF from the two crude oils, but not in fish exposed to WAF from diesel. Exposure to diesel appeared to induce oxidative stress to a larger extent than exposure to crude oils. Other biomarkers (glutathione S-transferases, acetylcholine esterase, vitellogenin) did not appear to respond to the exposure and hence did not discriminate among oils. Biomarker responses in cod after exposure to weathered crude oils and diesel suggested the CYP1A system and oxidative stress markers to have the highest potential for discriminating among different oil types and to monitor the environmental consequences of spills. PY 2014 PD SEP SO Aquatic Toxicology SN 0166-445X PU Elsevier Science Bv VL 154 UT 000340339800027 BP 240 EP 252 DI 10.1016/j.aquatox.2014.05.013 ID 30131 ER EF