An ELISA technique for rapid diagnosis of vibriosis in sea bass Dicentrarchus labrax

Type Article
Date 1993-03
Language English
Author(s) Romestand B1, Dragesco A1, Breuil Gilles2, Coste F1, Bouix G1
Affiliation(s) 1 : UNIV MONTPELLIER 2, PARASITOL & IMMUNOL LAB, CASE 096, PL EUGENE BATAILLON, F-34095 MONTPELLIER 05, FRANCE.
2 : IFREMER, GIE RECH AQUACOLE, EQUIPE PATHOL, F-34250 PALAVAS LES FLOTS, FRANCE.
Source Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1993-03 , Vol. 15 , N. 2 , P. 137-143
DOI 10.3354/dao015137
WOS© Times Cited 10
Abstract Three batches of rabbits were immunized against 2 strains of Vibrio anguillarum (Va V 62 and Va V 408), which cause vibriosis in sea bass Dicentrarchus labrax (L. 1758) Serranidae. Homologous and heterologous reactions were obtained against these Vibrio strains and other bacteria using an improved indirect ELISA technique. Antibody and antigen titrations revealed high immunogenecity (high antibody titers for the antisera) of the 2 Vibrio strains. They correspond to serotype I and show close affinity. The specificity and sensitivity of 1 selected antiserum were tested on brain extracts from healthy sea bass and those infected artificially with V. anguillarum serotypes I and III. Preliminary results confirm the reliability of the technique and warrant its use for improving disease diagnosis.
Full Text
File Pages Size Access
Publisher's official version 7 549 KB Open access
Top of the page