FN Archimer Export Format PT J TI An ELISA technique for rapid diagnosis of vibriosis in sea bass Dicentrarchus labrax BT AF ROMESTAND, B DRAGESCO, A BREUIL, Gilles COSTE, F BOUIX, G AS 1:1;2:1;3:2;4:1;5:1; FF 1:;2:;3:;4:;5:; C1 UNIV MONTPELLIER 2, PARASITOL & IMMUNOL LAB, CASE 096, PL EUGENE BATAILLON, F-34095 MONTPELLIER 05, FRANCE. IFREMER, GIE RECH AQUACOLE, EQUIPE PATHOL, F-34250 PALAVAS LES FLOTS, FRANCE. C2 UNIV MONTPELLIER, FRANCE IFREMER, FRANCE SI PALAVAS IN WOS Ifremer jusqu'en 2018 IF 1.556 TC 10 UR https://archimer.ifremer.fr/doc/00193/30400/28992.pdf LA English DT Article AB Three batches of rabbits were immunized against 2 strains of Vibrio anguillarum (Va V 62 and Va V 408), which cause vibriosis in sea bass Dicentrarchus labrax (L. 1758) Serranidae. Homologous and heterologous reactions were obtained against these Vibrio strains and other bacteria using an improved indirect ELISA technique. Antibody and antigen titrations revealed high immunogenecity (high antibody titers for the antisera) of the 2 Vibrio strains. They correspond to serotype I and show close affinity. The specificity and sensitivity of 1 selected antiserum were tested on brain extracts from healthy sea bass and those infected artificially with V. anguillarum serotypes I and III. Preliminary results confirm the reliability of the technique and warrant its use for improving disease diagnosis. PY 1993 PD MAR SO Diseases Of Aquatic Organisms SN 0177-5103 PU Inter-research VL 15 IS 2 UT A1993LE60500008 BP 137 EP 143 DI 10.3354/dao015137 ID 30400 ER EF