Direct and fast detection of Alexandrium minutum algae by using high frequency microbalance

Type Article
Date 2014-09
Language English
Author(s) Sousa Celia1, 2, 3, Compere ChantalORCID4, Dreanno CatherineORCID4, Crassous Marie-Pierre4, Gas Fabienne5, Baus Beatrice5, Perrot Hubert1, 2
Affiliation(s) 1 : Univ Paris 06, Univ Paris 04, UMR 8235, Lab Interfaces & Syst Electrochim, F-75252 Paris, France.
2 : CNRS, UMR 8235, LISE, F-75005 Paris, France.
3 : Univ Fed Rio de Janeiro, Inst Quim, BR-21941909 Rio De Janeiro, Brazil.
4 : IFREMER, Ctr Brest, Lab Detect Capteurs & Mesures, F-29280 Plouzane, France.
5 : CEA Marcoule, Direct Sci Vivant, IBEB SBTN LDCAE, F-30200 Bagnols Sur Ceze, France.
Source Journal Of Microbiological Methods (0167-7012) (Elsevier Science Bv), 2014-09 , Vol. 103 , P. 49-54
DOI 10.1016/j.mimet.2014.05.025
WOS© Times Cited 8
Keyword(s) Alexandrium minutum, Quartz microbalance, Microalgae, Immunosensor
Abstract In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes.
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