TY - JOUR T1 - Direct and fast detection of Alexandrium minutum algae by using high frequency microbalance A1 - Sousa,Celia A1 - Compere,Chantal A1 - Dreanno,Catherine A1 - Crassous,Marie-Pierre A1 - Gas,Fabienne A1 - Baus,Beatrice A1 - Perrot,Hubert AD - Univ Paris 06, Univ Paris 04, UMR 8235, Lab Interfaces & Syst Electrochim, F-75252 Paris, France. AD - CNRS, UMR 8235, LISE, F-75005 Paris, France. AD - Univ Fed Rio de Janeiro, Inst Quim, BR-21941909 Rio De Janeiro, Brazil. AD - IFREMER, Ctr Brest, Lab Detect Capteurs & Mesures, F-29280 Plouzane, France. AD - CEA Marcoule, Direct Sci Vivant, IBEB SBTN LDCAE, F-30200 Bagnols Sur Ceze, France. UR - https://doi.org/10.1016/j.mimet.2014.05.025 DO - 10.1016/j.mimet.2014.05.025 KW - Alexandrium minutum KW - Quartz microbalance KW - Microalgae KW - Immunosensor N2 - In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes. Y1 - 2014/09 PB - Elsevier Science Bv JF - Journal Of Microbiological Methods SN - 0167-7012 VL - 103 SP - 49 EP - 54 ID - 30492 ER -