FN Archimer Export Format
PT J
TI Direct and fast detection of Alexandrium minutum algae by using high frequency microbalance
BT 
AF SOUSA, Celia
   COMPERE, Chantal
   DREANNO, Catherine
   CRASSOUS, Marie-Pierre
   GAS, Fabienne
   BAUS, Beatrice
   PERROT, Hubert
AS 1:1,2,3;2:4;3:4;4:4;5:5;6:5;7:1,2;
FF 1:;2:PDG-REM-RDT;3:PDG-REM-RDT-LDCM;4:PDG-REM-RDT-LDCM;5:;6:;7:;
C1 Univ Paris 06, Univ Paris 04, UMR 8235, Lab Interfaces & Syst Electrochim, F-75252 Paris, France.
   CNRS, UMR 8235, LISE, F-75005 Paris, France.
   Univ Fed Rio de Janeiro, Inst Quim, BR-21941909 Rio De Janeiro, Brazil.
   IFREMER, Ctr Brest, Lab Detect Capteurs & Mesures, F-29280 Plouzane, France.
   CEA Marcoule, Direct Sci Vivant, IBEB SBTN LDCAE, F-30200 Bagnols Sur Ceze, France.
C2 UNIV PARIS 06, FRANCE
   CNRS, FRANCE
   UNIV FED RIO DE JANEIRO, BRAZIL
   IFREMER, FRANCE
   CEA, FRANCE
SI BREST
SE PDG-REM-RDT
   PDG-REM-RDT-LDCM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 2.026
TC 8
UR https://archimer.ifremer.fr/doc/00194/30492/28897.pdf
LA English
DT Article
DE ;Alexandrium minutum;Quartz microbalance;Microalgae;Immunosensor
AB In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes.
PY 2014
PD SEP
SO Journal Of Microbiological Methods
SN 0167-7012
PU Elsevier Science Bv
VL 103
UT 000340986800008
BP 49
EP 54
DI 10.1016/j.mimet.2014.05.025
ID 30492
ER

EF