FN Archimer Export Format
PT J
TI Genotoxicity and activation of cellular defenses in transplanted zebra mussels Dreissena polymorpha along the Seine river
BT 
AF CHATEL, Annelle
   FAUCET-MARQUIS, Virginie
   GOURLAY-FRANCE, Catherine
   PFOHL-LESZKOWICZ, Annie
   HUBERT, Francoise
AS 1:1;2:2;3:1;4:2;5:1;
FF 1:;2:;3:;4:;5:;
C1 Irstea, Unite Rech Hydrosyst & Bioproc, F-92761 Antony, France.
   Univ Toulouse, INPT, Dept Bioproc & Syst Microbiens, ENSAT,Lab Genie Chim,CNRS,UMR 5503, F-31320 Auzeville Tolosane, France.
C2 IRSTEA, FRANCE
   UNIV TOULOUSE, FRANCE
IF 3.13
TC 19
UR https://archimer.ifremer.fr/doc/00195/30639/32450.pdf
LA English
DT Article
DE ;Biomarker;Pollution;DNA adducts;Detoxification;Dreissena polymorpha;RT-PCR
AB The aim of the present study was to confirm the relevance of studying DNA adduct formation in a field study. In that context, freshwater mussels Dreissena polymorpha, collected in a reference station, were transplanted in different sites with a pollution gradient. After one and two months, mussels were collected and DNA adduct formation was analyzed using the 32P post labelling technique on both gills and digestive glands. In addition, the expression of genes involved in the detoxification system (catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), HSP70, aryl hydrocarbon receptor (AHR), P glycoprotein (PgP), metallothionein (MT)) was assessed by RT-PCR. DNA adducts were observed at amount comparable to data from literature. Increase of DNA adducts after two months of transplantation could be correlated with strong modulation of gene expression implicated in detoxification processes. Indeed, PgP and HSP70 gene expressions were similarly induced in gills and digestive glands while SOD and CAT expressions were down regulated in both tissues. AHR, GST and MT genes were differently regulated depending upon the tissue studied and the level of contamination in the different sites. We demonstrated that mussels transplanted in the different stations with pollution gradient were able to biotransform PAHs, assessed by DNA adduct formation and the high decrease of detoxification genes. Specific DNA adducts pattern obtained after one and two month mussel transplantations demonstrated the relevance of DNA adduct as biomarker of environmental pollution.
PY 2015
PD APR
SO Ecotoxicology And Environmental Safety
SN 0147-6513
PU Academic Press Inc Elsevier Science
VL 114
UT 000350928800031
BP 241
EP 249
DI 10.1016/j.ecoenv.2014.03.023
ID 30639
ER

EF