Monoclonal antibodies against sporangia and spores of Marteilia sp. (Protozoa: Ascetospora)

Type Article
Date 1994-04
Language English
Author(s) Robledo Jaf1, Boulo VivianeORCID2, Mialhe Eric2, Despres Béatrice2, Figueras Antonio1
Affiliation(s) 1 : CSIC,INST INVEST MARINAS,DEPT BIOL & PATOL ORGANISMOS MARINOS,EDUARDO CABELLO 6,E-36208 VIGO,SPAIN.
2 : UNIV MONTPELLIER 2,IFREMER,CNRS,UMR 9947,DEF RESISTANCE INVERTEBRES MARINS,F-34095 MONTPELLIER 5,FRANCE.
Source Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1994-04 , Vol. 18 , N. 3 , P. 211-216
DOI 10.3354/dao018211
WOS© Times Cited 10
Keyword(s) Marteilia sp., Mytilus edulis, Monoclonal antibodies
Abstract Digestive glands of mussels Mytilus edulis from Brittany, France, infected with Marteilia sp. (Ascetospora) were used to purify the parasite. A modification of a previously used purification protocol increased purification efficiency, permitting sporangial primordia and sporangia of Marteilia sp. to be obtained. Mouse (Balb/c) monoclonal antibodies were generated against this parasite. From the fusion, 26 monoclonal antibodies against Marteilia sp. were obtained. Antibodies from 6 clones reacted only with Marteilia sp. cells and not with normal host tissues. Four of these antibodies (1/1-3, 3/1-1, 4/1-1 and 6/2-3) reacted with the sporangia wall and two with the spore cytoplasm (9/1-1 and 12/5-1). Antibodies cross-reacted with Marteilia refringens from Mytilus galloprovincialis obtained in the Ria de Vigo, Spain.
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