|Author(s)||Robledo Jaf1, Boulo Viviane2, Mialhe Eric2, Despres Béatrice2, Figueras Antonio1|
|Affiliation(s)||1 : CSIC,INST INVEST MARINAS,DEPT BIOL & PATOL ORGANISMOS MARINOS,EDUARDO CABELLO 6,E-36208 VIGO,SPAIN.
2 : UNIV MONTPELLIER 2,IFREMER,CNRS,UMR 9947,DEF RESISTANCE INVERTEBRES MARINS,F-34095 MONTPELLIER 5,FRANCE.
|Source||Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1994-04 , Vol. 18 , N. 3 , P. 211-216|
|WOS© Times Cited||10|
|Keyword(s)||Marteilia sp., Mytilus edulis, Monoclonal antibodies|
|Abstract||Digestive glands of mussels Mytilus edulis from Brittany, France, infected with Marteilia sp. (Ascetospora) were used to purify the parasite. A modification of a previously used purification protocol increased purification efficiency, permitting sporangial primordia and sporangia of Marteilia sp. to be obtained. Mouse (Balb/c) monoclonal antibodies were generated against this parasite. From the fusion, 26 monoclonal antibodies against Marteilia sp. were obtained. Antibodies from 6 clones reacted only with Marteilia sp. cells and not with normal host tissues. Four of these antibodies (1/1-3, 3/1-1, 4/1-1 and 6/2-3) reacted with the sporangia wall and two with the spore cytoplasm (9/1-1 and 12/5-1). Antibodies cross-reacted with Marteilia refringens from Mytilus galloprovincialis obtained in the Ria de Vigo, Spain.|
Robledo Jaf, Boulo Viviane, Mialhe Eric, Despres Béatrice, Figueras Antonio (1994). Monoclonal antibodies against sporangia and spores of Marteilia sp. (Protozoa: Ascetospora). Diseases Of Aquatic Organisms, 18(3), 211-216. Publisher's official version : https://doi.org/10.3354/dao018211 , Open Access version : https://archimer.ifremer.fr/doc/00196/30730/