Adult somatic progenitor cells and hematopoiesis in oysters

Type Article
Date 2014-09
Language English
Author(s) Jemaa Mohamed1, 2, 3, Morin Nathalie1, 2, 3, Cavelier Patricia1, 2, 4, Cau Julien1, 2, 5, Strub Jean Marc6, 7, Delsert Claude1, 2, 3, 8
Affiliation(s) 1 : Univ Montpellier 2, F-34095 Montpellier, France.
2 : Univ Montpellier I, F-34095 Montpellier, France.
3 : CRBM CNRS UMR 5237, F-34293 Montpellier, France.
4 : IGMM CNRS UMR 5535, F-34293 Montpellier, France.
5 : IGH CNRS UPR 1142, F-34396 Montpellier, France.
6 : Univ Strasbourg, F-67081 Strasbourg, France.
7 : IPHC CNRS UMR7178, F-67037 Strasbourg, France.
8 : IFREMER, LGP, F-17390 La Tremblade, France.
Source Journal Of Experimental Biology (0022-0949) (Company Of Biologists Ltd), 2014-09 , Vol. 217 , N. 17 , P. 3067-3077
DOI 10.1242/jeb.106575
WOS© Times Cited 32
Keyword(s) Hematopoiesis, Adult somatic progenitor cells, Hemocytes, Mollusk, Marine invertebrates
Abstract Long-lived animals show a non-observable age-related decline in immune defense, which is provided by blood cells that derive from self-renewing stem cells. The oldest living animals are bivalves. Yet, the origin of hemocytes, the cells involved in innate immunity, is unknown in bivalves and current knowledge about mollusk adult somatic stem cells is scarce. Here we identify a population of adult somatic precursor cells and show their differentiation into hemocytes. Oyster gill contains an as yet unreported irregularly folded structure (IFS) with stem-like cells bathing into the hemolymph. BrdU labeling revealed that the stem-like cells in the gill epithelium and in the nearby hemolymph replicate DNA. Proliferation of this cell population was further evidenced by phosphorylated-histone H3 mitotic staining. Finally, these small cells most abundant in the IFS epithelium were found positive for the stemness marker Sox2. We provide evidence for hematopoiesis by showing that co-expression of Sox2 and Cu/Zn SOD, a hemocyte-specific enzyme, does not occur in the gill epithelial cells but rather in the underlying tissues and vessels. We further confirm the hematopoietic features of these cells by the detection of Filamin, a protein specific for a sub-population of hemocytes, in large BrdU-labeled cells bathing into gill vessels. Altogether, our data show that progenitor cells differentiate into hemocytes in gill, which suggests that hematopoiesis occurs in oyster gills.
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