FN Archimer Export Format
PT J
TI Adult somatic progenitor cells and hematopoiesis in oysters
BT 
AF JEMAA, Mohamed
   MORIN, Nathalie
   CAVELIER, Patricia
   CAU, Julien
   STRUB, Jean Marc
   DELSERT, Claude
AS 1:1,2,3;2:1,2,3;3:1,2,4;4:1,2,5;5:6,7;6:1,2,3,8;
FF 1:;2:;3:;4:;5:;6:PDG-RBE-SG2M-LGPMM;
C1 Univ Montpellier 2, F-34095 Montpellier, France.
   Univ Montpellier I, F-34095 Montpellier, France.
   CRBM CNRS UMR 5237, F-34293 Montpellier, France.
   IGMM CNRS UMR 5535, F-34293 Montpellier, France.
   IGH CNRS UPR 1142, F-34396 Montpellier, France.
   Univ Strasbourg, F-67081 Strasbourg, France.
   IPHC CNRS UMR7178, F-67037 Strasbourg, France.
   IFREMER, LGP, F-17390 La Tremblade, France.
C2 UNIV MONTPELLIER, FRANCE
   UNIV MONTPELLIER, FRANCE
   CNRS, FRANCE
   CNRS, FRANCE
   CNRS, FRANCE
   UNIV STRASBOURG, FRANCE
   CNRS, FRANCE
   IFREMER, FRANCE
SI MONTPELLIER
SE PDG-RBE-SG2M-LGPMM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.897
TC 58
UR https://archimer.ifremer.fr/doc/00197/30802/29172.pdf
LA English
DT Article
DE ;Hematopoiesis;Adult somatic progenitor cells;Hemocytes;Mollusk;Marine invertebrates
AB Long-lived animals show a non-observable age-related decline in immune defense, which is provided by blood cells that derive from self-renewing stem cells. The oldest living animals are bivalves. Yet, the origin of hemocytes, the cells involved in innate immunity, is unknown in bivalves and current knowledge about mollusk adult somatic stem cells is scarce. Here we identify a population of adult somatic precursor cells and show their differentiation into hemocytes. Oyster gill contains an as yet unreported irregularly folded structure (IFS) with stem-like cells bathing into the hemolymph. BrdU labeling revealed that the stem-like cells in the gill epithelium and in the nearby hemolymph replicate DNA. Proliferation of this cell population was further evidenced by phosphorylated-histone H3 mitotic staining. Finally, these small cells most abundant in the IFS epithelium were found positive for the stemness marker Sox2. We provide evidence for hematopoiesis by showing that co-expression of Sox2 and Cu/Zn SOD, a hemocyte-specific enzyme, does not occur in the gill epithelial cells but rather in the underlying tissues and vessels. We further confirm the hematopoietic features of these cells by the detection of Filamin, a protein specific for a sub-population of hemocytes, in large BrdU-labeled cells bathing into gill vessels. Altogether, our data show that progenitor cells differentiate into hemocytes in gill, which suggests that hematopoiesis occurs in oyster gills.
PY 2014
PD SEP
SO Journal Of Experimental Biology
SN 0022-0949
PU Company Of Biologists Ltd
VL 217
IS 17
UT 000342505700019
BP 3067
EP 3077
DI 10.1242/jeb.106575
ID 30802
ER

EF