FN Archimer Export Format
PT J
TI Outer membrane vesicles are vehicles for the delivery of Vibrio tasmaniensis virulence factors to oyster immune cells
BT 
AF VANHOVE, Audrey
   DUPERTHUY, Marylise
   CHARRIERE, Guillaume
   LE ROUX, Frederique
   GOUDENEGE, David
   GOURBAL, Benjamin
   KIEFFER-JAQUINOD, Sylvie
   COUTE, Yohann
   WAI, Sun Nyunt
   DESTOUMIEUX-GARZON, Delphine
AS 1:1,2,3,4,5;2:2,6;3:1,2,3,4,5;4:7,8,9;5:7,8,9;6:10;7:11,12,13;8:11,12,13;9:6;10:1,2,3,4,5;
FF 1:;2:PDG-RBE-BOME-LALR;3:;4:PDG-RBE-PFOM;5:PDG-RBE-PFOM;6:;7:;8:;9:;10:;
C1 CNRS, UMR 5119, Ecol Coastal Marine Syst, F-34095 Montpellier, France.
   IFREMER, Ecol Coastal Marine Syst, F-34095 Montpellier, France.
   Univ Montpellier I, Ecol Coastal Marine Syst, F-34095 Montpellier, France.
   Univ Montpellier 2, Ecol Coastal Marine Syst, F-34095 Montpellier, France.
   IRD, F-34095 Montpellier, France.
   Umea Univ, Dept Mol Biol, Lab Mol Infect Med Sweden MIMS, S-90187 Umea, Sweden.
   IFREMER, Unite Physiol Fonct Organismes Marins, F-29280 Plouzane, France.
   Univ Paris 04, Univ Paris 06, Integrat Biol Marine Models, F-29688 Roscoff, France.
   CNRS, Stn Biol Roscoff, UMR 8227, Integrat Biol Marine Models, F-29688 Roscoff, France.
   Univ Perpignan, CNRS, UMR 5244, Ecol & Evolut Interact, F-66860 Perpignan, France.
   Univ Grenoble Alpes, U1038, F-38054 Grenoble, France.
   CEA Grenoble, Biol Grande Echelle, IRTSV, F-38054 Grenoble, France.
   INSERM, U1038, F-38054 Grenoble, France.
C2 CNRS, FRANCE
   IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
   UNIV MONTPELLIER, FRANCE
   IRD, FRANCE
   UNIV UMEA, SWEDEN
   IFREMER, FRANCE
   UNIV PARIS 04, FRANCE
   CNRS, FRANCE
   UNIV PERPIGNAN, FRANCE
   UNIV GRENOBLE, FRANCE
   CEA, FRANCE
   INSERM, FRANCE
SI MONTPELLIER
   ROSCOFF
SE PDG-RBE-BOME-LALR
   PDG-RBE-PFOM
UM IHPE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-europe
   copubli-univ-france
IF 5.932
TC 56
UR https://archimer.ifremer.fr/doc/00199/31009/29459.pdf
LA English
DT Article
AB Vibrio tasmaniensis LGP32, a facultative intracellular pathogen of oyster haemocytes, was shown here to release outer membrane vesicles (OMVs) both in the extracellular milieu and inside haemocytes. Intracellular release of OMVs occurred inside phagosomes of intact haemocytes having phagocytosed few vibrios as well as in damaged haemocytes containing large vacuoles heavily loaded with LGP32. The OMV proteome of LGP32 was shown to be rich in hydrolases (25%) including potential virulence factors such as proteases, lipases, phospholipases, haemolysins and nucleases. One major caseinase/gelatinase named Vsp for vesicular serine protease was found to be specifically secreted through OMVs in which it is enclosed. Vsp was shown to participate in the virulence phenotype of LGP32 in oyster experimental infections. Finally, OMVs were highly protective against antimicrobial peptides, increasing the minimal inhibitory concentration of polymyxin B by 16-fold. Protection was conferred by OMV titration of polymyxin B but did not depend on the activity of Vsp or another OMV-associated protease. Altogether, our results show that OMVs contribute to the pathogenesis of LGP32, being able to deliver virulence factors to host immune cells and conferring protection against antimicrobial peptides.
PY 2015
PD APR
SO Environmental Microbiology
SN 1462-2912
PU Wiley-blackwell
VL 17
IS 4
UT 000352545100019
BP 1152
EP 1165
DI 10.1111/1462-2920.12535
ID 31009
ER

EF