Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1

Type Article
Date 2014-07
Language English
Author(s) Segarra Amelie1, Mauduit Florian1, Faury Nicole1, Trancart Suzanne1, Degremont LionelORCID, Tourbiez Delphine1, Haffner Philippe1, Barbosa-Solomieu Valerie1, Pepin Jean-Francois2, Travers Marie-AgnesORCID1, Renault Tristan1
Affiliation(s) 1 : Ifremer, Inst Francais Rech Exploitat Mer, Unite Sante Genet & Microbiol Mollusques SG2M, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France.
2 : IFREMER, Lab Environm Ressources Pertuis Charentais, F-17390 La Tremblade, France.
Source Bmc Genomics (1471-2164) (Biomed Central Ltd), 2014-07 , Vol. 15 , N. 580 , P. 1-13
DOI 10.1186/1471-2164-15-580
WOS© Times Cited 51
Keyword(s) Interactions, Ostreid herpesvirus 1, Gene expression, Susceptibility, Crassostrea gigas
Abstract Background:
Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches.

The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant genetic component. Two families with contrasted survival rates were selected. A total of 39 viral genes and five host genes were monitored by real-time PCR. Initial results provided information on (i) the virus cycle of OsHV-1 based on the kinetics of viral DNA replication and transcription and (ii) host defense mechanisms against the virus.

In the two selected families, the detected amounts of viral DNA and RNA were significantly different. This result suggests that Pacific oysters are genetically diverse in terms of their susceptibility to OsHV-1 infection. This contrasted susceptibility was associated with dissimilar host gene expression profiles. Moreover, the present study showed a positive correlation between viral DNA amounts and the level of expression of selected oyster genes.
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Segarra Amelie, Mauduit Florian, Faury Nicole, Trancart Suzanne, Degremont Lionel, Tourbiez Delphine, Haffner Philippe, Barbosa-Solomieu Valerie, Pepin Jean-Francois, Travers Marie-Agnes, Renault Tristan (2014). Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1. Bmc Genomics, 15(580), 1-13. Publisher's official version : https://doi.org/10.1186/1471-2164-15-580 , Open Access version : https://archimer.ifremer.fr/doc/00199/31029/