FN Archimer Export Format
PT J
TI Gonad transcriptome analysis of pearl oyster Pinctada margaritifera: identification of potential sex differentiation and sex determining genes
BT 
AF TEANINIURAITEMOANA, Vaihiti
   HUVET, Arnaud
   LEVY, Peva
   KLOPP, Christophe
   LHUILLIER, Emeline
   GAERTNER-MAZOUNI, Nabila
   GUEGUEN, Yannick
   LE MOULLAC, Gilles
AS 1:1;2:2;3:1;4:3;5:4;6:5;7:1;8:1;
FF 1:PDG-RBE-RMPF;2:PDG-RBE-PFOM-PI;3:PDG-RBE-RMPF;4:;5:;6:;7:PDG-RBE-BOME-LALR;8:PDG-RBE-RMPF;
C1 Ifremer, UMR 241 EIO, Labex CORAIL, BP 7004, 98719 Taravao, Tahiti, Polynésie Française.
   IFREMER, UMR 6539, LEMAR, F-29280 Plouzane, France.
   INRA, UR875, F-31326 Castanet Tolosan, France.
   INRA, GeT PlaGe, F-31326 Castanet Tolosan, France.
   Univ Polynesie Francaise, UMR 241, EIO, F-98702 Faaa, Tahiti, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   INRA, FRANCE
   INRA, FRANCE
   UNIV POLYNESIE FRANCAISE, FRANCE
SI TAHITI
   BREST
   MONTPELLIER
SE PDG-RBE-RMPF
   PDG-RBE-PFOM-PI
   PDG-RBE-BOME-LALR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
IF 3.986
TC 71
UR https://archimer.ifremer.fr/doc/00201/31194/29593.pdf
LA English
DT Article
DE ;Pinctada margaritifera;Gametogenesis;Transcriptome;Differential expression;Sex determinism
AB Background  Black pearl farming is based on culture of the blacklip pearl oyster Pinctada margaritifera (Mollusca, lophotrochozoa), a protandrous hermaphrodite species. At first maturation, all individuals are males. The female sex appears progressively from two years old, which represents a limitation for broodstock conditioning for aquaculture production. In marine mollusks displaying hermaphroditic features, data on sexual determinism and differentiation, including the molecular sex determining cascade, are scarce. To increase genomic resources and identify the molecular mechanisms whereby gene expression may act in the sexual dimorphism of P. margaritifera, we performed gonad transcriptome analysis.  Results  The gonad transcriptome of P. margaritifera was sequenced from several gonadic samples of males and females at different development stages, using a Next-Generation-Sequencing method and RNAseq technology. After Illumina sequencing, assembly and annotation, we obtained 70,147 contigs of which 62.2% shared homologies with existing protein sequences, and 9% showed functional annotation with Gene Ontology terms. Differential expression analysis identified 1,993 differentially expressed contigs between the different categories of gonads. Clustering methods of samples revealed that the sex explained most of the variation in gonad gene expression. K-means clustering of differentially expressed contigs showed 815 and 574 contigs were more expressed in male and female gonads, respectively. The analysis of these contigs revealed the presence of known specific genes coding for proteins involved in sex determinism and/or differentiation, such as dmrt and fem-1 like for males, or foxl2 and vitellogenin for females. The specific gene expression profiles of pmarg-fem1-like, pmarg-dmrt and pmarg-foxl2 in different reproductive stages (undetermined, sexual inversion and regression) suggest that these three genes are potentially involved in the sperm-oocyte switch in P. margaritifera.  Conclusions  The study provides a new transcriptomic tool to study reproduction in hermaphroditic marine mollusks. It identifies sex differentiation and potential sex determining genes in P. margaritifera, a protandrous hermaphrodite species.
PY 2014
PD JUL
SO Bmc Genomics
SN 1471-2164
PU Biomed Central Ltd
VL 15
IS 491
UT 000338246900001
BP 1
EP 20
DI 10.1186/1471-2164-15-491
ID 31194
ER

EF