TY - JOUR T1 - Microorganisms persist at record depths in the subseafloor of the Canterbury Basin A1 - Ciobanu,Maria Cristina A1 - Burgaud,Gaetan A1 - Dufresne,Alexis A1 - Breuker,Anja A1 - Redou,Vanessa A1 - Ben Maamar,Sarah A1 - Gaboyer,Frederic A1 - Vandenabeele-Trambouze,Odile A1 - Lipp,Julius Sebastian A1 - Schippers,Axel A1 - Vandenkoornhuyse,Philippe A1 - Barbier,Georges A1 - Jebbar,Mohamed A1 - Godfroy,Anne A1 - Alain,Karine AD - UBO, UEB, IUEM, UMR 6197,LMEE, Plouzane, France. AD - IUEM, CNRS, UMR 6197, LMEE, Plouzane, France. AD - IFREMER, UMR6197, LMEE, Plouzane, France. AD - Univ Brest, UEB, SFR ScInBioS IFR148,ESIAB, Lab Univ Biodiversite & Ecol Microbienne EA 3882, Plouzane, France. AD - Univ Rennes 1, CNRS, UMR 6553, ECOBIO, Rennes, France. AD - BGR, Hannover, Germany. AD - Univ Bremen, Dept Geosci, Organ Geochem Grp, D-28359 Bremen, Germany. AD - Univ Bremen, MARUM Ctr Marine Environm Sci, D-28359 Bremen, Germany. UR - https://doi.org/10.1038/ismej.2013.250 DO - 10.1038/ismej.2013.250 KW - deep biosphere KW - subsurface life KW - eukaryote KW - record depth N2 - The subsurface realm is colonized by microbial communities to depths of >1000 meters below the seafloor (m.b.sf.), but little is known about overall diversity and microbial distribution patterns at the most profound depths. Here we show that not only Bacteria and Archaea but also Eukarya occur at record depths in the subseafloor of the Canterbury Basin. Shifts in microbial community composition along a core of nearly 2 km reflect vertical taxa zonation influenced by sediment depth. Representatives of some microbial taxa were also cultivated using methods mimicking in situ conditions. These results suggest that diverse microorganisms persist down to 1922 m.b.sf. in the seafloor of the Canterbury Basin and extend the previously known depth limits of microbial evidence (i) from 159 to 1740 m.b.sf. for Eukarya and (ii) from 518 to 1922 m.b.sf. for Bacteria. Y1 - 2014/07 PB - Nature Publishing Group JF - Isme Journal SN - 1751-7362 VL - 8 IS - 7 SP - 1370 EP - 1380 ID - 31243 ER -