Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas

Type Article
Date 2014-08
Language English
Author(s) Bigot Laetitia1, 2, Beets Isabel3, Dubos Marie-Pierre1, 2, Boudry PierreORCID4, Schoofs Liliane3, Favrel Pascal1, 2
Affiliation(s) 1 : Univ Caen Basse Normandie, Biol Organismes Aquat & Ecosyst BOREA, F-14032 Caen 05, France.
2 : CNRS, BOREA, UMR 7208, F-14032 Caen 05, France.
3 : Katholieke Univ Leuven, Dept Biol, Funct Genom & Prote Grp, B-3000 Louvain, Belgium.
4 : IFREMER, UMR 6539, LEMAR, Unite Physiol Fonct Organismes Marins, F-29280 Plouzane, France.
Source Journal Of Experimental Biology (0022-0949) (Company Of Biologists Ltd), 2014-08 , Vol. 217 , N. 16 , P. 2974-2982
DOI 10.1242/jeb.104067
WOS© Times Cited 18
Keyword(s) Oyster, Crassostrea gigas, sNPF, Energy metabolism, Reproduction, Starvation
Abstract Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the identification of a receptor (CgsNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) of insects. A reverse endocrinology approach was undertaken to identify the peptide ligand(s) of this orphan receptor. Though structurally distinct from insect sNPFs, three RFamide peptides derived from the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, specifically activate the receptor in a dose-dependent manner, with respective EC50 values (halfmaximal effective concentrations) of 1.1, 2.1 and 4.1 µmol l-1. We found that both Cg-sNPFR-like receptor and LFRFamide encoding transcripts are expressed in the oyster central nervous system and in other tissues as well, albeit at lower levels. Mass spectrometry analysis confirmed the wide distribution of LFRFamide mature peptides in several central and peripheral tissues. The Cg-sNPFRlike receptor was more abundantly expressed in ganglia of females than of males, and upregulated in starved oysters. In the gonad area, highest receptor gene expression occurred at the start of gametogenesis, when storage activity is maximal. Our results suggest that signaling of LFRFamide peptides through the CgsNPFR-like receptor might play a role in the coordination of nutrition, energy storage and metabolism in C. gigas, possibly by promoting storage at the expense of reproduction.
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Bigot Laetitia, Beets Isabel, Dubos Marie-Pierre, Boudry Pierre, Schoofs Liliane, Favrel Pascal (2014). Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas. Journal Of Experimental Biology, 217(16), 2974-2982. Publisher's official version : https://doi.org/10.1242/jeb.104067 , Open Access version : https://archimer.ifremer.fr/doc/00207/31777/