FN Archimer Export Format
PT J
TI Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas
BT 
AF BIGOT, Laetitia
   BEETS, Isabel
   DUBOS, Marie-Pierre
   BOUDRY, Pierre
   SCHOOFS, Liliane
   FAVREL, Pascal
AS 1:1,2;2:3;3:1,2;4:4;5:3;6:1,2;
FF 1:PDG-RBE-PFOM-PI;2:;3:;4:PDG-RBE-PFOM;5:;6:;
C1 Univ Caen Basse Normandie, Biol Organismes Aquat & Ecosyst BOREA, F-14032 Caen 05, France.
   CNRS, BOREA, UMR 7208, F-14032 Caen 05, France.
   Katholieke Univ Leuven, Dept Biol, Funct Genom & Prote Grp, B-3000 Louvain, Belgium.
   IFREMER, UMR 6539, LEMAR, Unite Physiol Fonct Organismes Marins, F-29280 Plouzane, France.
C2 UNIV CAEN, FRANCE
   CNRS, FRANCE
   UNIV KATHOLIEKE LEUVEN, BELGIUM
   IFREMER, FRANCE
SI BREST
SE PDG-RBE-PFOM-PI
   PDG-RBE-PFOM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 2.897
TC 24
UR https://archimer.ifremer.fr/doc/00207/31777/30188.pdf
LA English
DT Article
DE ;Oyster;Crassostrea gigas;sNPF;Energy metabolism;Reproduction;Starvation
AB Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the identification of a receptor (CgsNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) of insects. A reverse endocrinology approach was undertaken to identify the peptide ligand(s) of this orphan receptor. Though structurally distinct from insect sNPFs, three RFamide peptides derived from the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, specifically activate the receptor in a dose-dependent manner, with respective EC50 values (halfmaximal effective concentrations) of 1.1, 2.1 and 4.1 µmol l-1. We found that both Cg-sNPFR-like receptor and LFRFamide encoding transcripts are expressed in the oyster central nervous system and in other tissues as well, albeit at lower levels. Mass spectrometry analysis confirmed the wide distribution of LFRFamide mature peptides in several central and peripheral tissues. The Cg-sNPFRlike receptor was more abundantly expressed in ganglia of females than of males, and upregulated in starved oysters. In the gonad area, highest receptor gene expression occurred at the start of gametogenesis, when storage activity is maximal. Our results suggest that signaling of LFRFamide peptides through the CgsNPFR-like receptor might play a role in the coordination of nutrition, energy storage and metabolism in C. gigas, possibly by promoting storage at the expense of reproduction.
PY 2014
PD AUG
SO Journal Of Experimental Biology
SN 0022-0949
PU Company Of Biologists Ltd
VL 217
IS 16
UT 000341190300024
BP 2974
EP 2982
DI 10.1242/jeb.104067
ID 31777
ER

EF