FN Archimer Export Format PT J TI Ostreid Herpesvirus 1 Infection among Pacific Oyster (Crassostrea gigas) Spat: Relevance of Water Temperature to Virus Replication and Circulation Prior to the Onset of Mortality BT AF RENAULT, Tristan BOUQUET, Anne Lise MAURICE, Julien-Thomas LUPO, Coralie BLACHIER, Philippe AS 1:1;2:2;3:2;4:1;5:2; FF 1:PDG-RBE-SG2M;2:;3:;4:PDG-RBE-SG2M-LGPMM;5:; C1 IFREMER, Unite Sante Genet & Microbiol Mollusques SG2M, Lab Genet & Pathol Mollusques Marins, La Tremblade, France. Ctr Reg Experimentat & Applicat Aquacole, Prise De Terdoux, Le Chateau Dole, France. C2 IFREMER, FRANCE CREAA, FRANCE SI LA TREMBLADE SE PDG-RBE-SG2M PDG-RBE-SG2M-LGPMM IN WOS Ifremer jusqu'en 2018 copubli-france IF 3.668 TC 62 UR https://archimer.ifremer.fr/doc/00207/31871/30320.pdf LA English DT Article AB number of bivalve species worldwide, including the Pacific oyster, Crassostrea gigas, have been affected by mass mortality events associated with herpesviruses, resulting in significant losses. A particular herpesvirus was purified from naturally infected larval Pacific oysters, and its genome was completely sequenced. This virus has been classified as Ostreid herpesvirus 1 (OsHV-1) within the family Malacoherpesviridae. Since 2008, mass mortality outbreaks among C. gigas in Europe have been related to the detection of a variant of OsHV-1 called µVar. Additional data are necessary to better describe mortality events in relation to environmental-parameter fluctuations and OsHV-1 detection. For this purpose, a single batch of Pacific oyster spat was deployed in 4 different locations in the Marennes-Oleron area (France): an oyster pond (“claire”), a shellfish nursery, and two locations in the field. Mortality rates were recorded based on regular observation, and samples were collected to search for and quantify OsHV-1 DNA by real-time PCR. Although similar massive mortality rates were reported at the 4 sites, mortality was detected earlier in the pond and in the nursery than at both field sites. This difference may be related to earlier increases in water temperature. Mass mortality was observed among oysters a few days after increases in the number of PCR-positive oysters and viral-DNA amounts were recorded. An initial increment in the number of PCR-positive oysters was reported at both field sites during the survey in the absence of significant mortality. During this period, the water temperature was below 16°C. PY 2014 PD SEP SO Applied And Environmental Microbiology SN 0099-2240 PU Amer Soc Microbiology VL 80 IS 17 UT 000341486500027 BP 5419 EP 5426 DI 10.1128/AEM.00484-14 ID 31871 ER EF