Temperature and Food Influence Shell Growth and Mantle Gene Expression of Shell Matrix Proteins in the Pearl Oyster Pinctada margaritifera

Type Article
Date 2014-08
Language English
Author(s) Joubert Caroline1, Linard Clementine1, 2, Le Moullac GillesORCID1, Soyez Claude1, Saulnier Denis1, Teaniniuraitemoana Vaihiti1, 2, Ky Chin-Long1, Gueguen YannickORCID1
Affiliation(s) 1 : Labex Corail, IFREMER, Ctr Pacifique, UMR Ecosyst Insulaires Oceaniens 241, Tahiti, France.
2 : Univ French Polynesia, UMR Ecosyst Insulaires Oceaniens 241, Tahiti, France.
Source Plos One (1932-6203) (Public Library Science), 2014-08 , Vol. 9 , N. 8 , P. e103944
DOI 10.1371/journal.pone.0103944
WOS© Times Cited 55
Abstract In this study, we analyzed the combined effect of microalgal concentration and temperature on the shell growth of the bivalve Pinctada margaritifera and the molecular mechanisms underlying this biomineralization process. Shell growth was measured after two months of rearing in experimental conditions, using calcein staining of the calcified structures. Molecular mechanisms were studied though the expression of 11 genes encoding proteins implicated in the biomineralization process, which was assessed in the mantle. We showed that shell growth is influenced by both microalgal concentration and temperature, and that these environmental factors also regulate the expression of most of the genes studied. Gene expression measurement of shell matrix protein thereby appears to be an appropriate indicator for the evaluation of the biomineralization activity in the pearl oyster P. margaritifera under varying environmental conditions. This study provides valuable information on the molecular mechanisms of mollusk shell growth and its environmental control.
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Joubert Caroline, Linard Clementine, Le Moullac Gilles, Soyez Claude, Saulnier Denis, Teaniniuraitemoana Vaihiti, Ky Chin-Long, Gueguen Yannick (2014). Temperature and Food Influence Shell Growth and Mantle Gene Expression of Shell Matrix Proteins in the Pearl Oyster Pinctada margaritifera. Plos One, 9(8), e103944. Publisher's official version : https://doi.org/10.1371/journal.pone.0103944 , Open Access version : https://archimer.ifremer.fr/doc/00210/32095/